2009年新型甲型H1N1流感病毒非结构蛋白基因进化分析

Genetic characteristics and deduced protein variation of nonstructural protein of novel influenza virus A/H1N1 in 2009 pandemic

目的:探讨2009年新型甲型H1N1流感病毒的非结构蛋白(NS)基因进化规律。方法:从NCBI下载2009年新型甲型H1N1流感病毒以及北美、欧洲、亚洲地区以往流行的甲型H1N1流感病毒NS基因序列,利用Molecular Evolutionary Genetics Analysis version 4.0(MEGA4.0)软件对所选序列进行基因进化分析,并用NJ法构建进化树;对2009年新型甲型H1N1流感病毒NS基因核苷酸序列同源性及编码蛋白氨基酸序列进行分析。结果:2009年新型甲型H1N1流感病毒NS基因来源于猪A/H1N1流感病毒,与2005～2007年猪A/H1N1流感病毒具有较高的同源性(97.5%～97.6%),与1930～2007年猪A/H1N1流感病毒具有明显的时间进化关系;其重要抗原及拮抗宿主抗病毒能力的氨基酸位点基本没有变异。结论:2009年新型甲型H1N1流感病毒NS基因来源于猪A/H1N1流感病毒,NS基因编码蛋白拮抗宿主抗病毒能力并没有改变。

Objective： To elucidate the genetic characteristics and deduced protein variation of nonstructural protein（NS） gene of the novel influenza virus A/H1N1 in 2009 pandemic. Methods： The sequence of NS gene of A/H1N1 viruses isolated in North America, Europe,and Asia during 1930-2009 were downloaded from NCBI database. MEGA4.0 software and NJ method were used for sequence alignment,protein sequence alignment,and the phylogenetic tree construction. Results： The NS genes of novel influenza virus A/H1N1 in 2009 pandemic were originated from A/swine/H1N1 virus of 2005-2007;they shared a high homology of 97.5%-97.6%. There was an obvious evolutionary relationship between the NS genes of novel A/H1N1 virus and those of the influenza A/swine/H1N1 viruses isolated in North America from 1930 to 2007. No obvious changes were found in the amino acid sites for the antagonistic function of NS1 against the host antiviral capacity among these viruses. Conclusion= The NS gene of novel influenza virus A/H1N1 in 2009 pandemic might evolve from swine A/H1N1 influenza viruses isolated in the United States. The antagonistic function of NS1 against the host antiviral capacity is not changed.