原花青素预处理对体外人源细胞辐射损伤的防护作用

Protective effects of proanthocyanidin on radiation-induced injury of human-derived cells

目的:探讨原花青素(PC)预处理对体外人淋巴母细胞AHH-1和肠腺上皮细胞HIEC电离辐射损伤的防护效果及可能的作用机制。方法:采用CCK-8法检测原花青素预处理对γ射线照射后AHH-1、HIEC细胞存活率的影响,Annexin-Ⅴ/PI染色和流式细胞术分析细胞凋亡及细胞周期的变化,蛋白质印迹法检测细胞Bcl-2蛋白表达变化。结果:PC预处理的细胞辐照后存活率明显升高(P<0.01),4Gy照射用药组AHH-1细胞凋亡率(11.78%)显著低于单纯照射组(26.38%,P<0.01),8Gy照射用药组HIEC细胞凋亡率(5.32%)亦显著低于单纯照射组(12.45%,P<0.01)。PC预处理组AHH-1、HIEC细胞受照射后其Bcl-2蛋白表达下降受到抑制。结论:PC预处理对细胞的辐射损伤具有较好的防护效果,可能与其促进受损细胞Bcl-2蛋白表达有关。

Objective：To investigate the protective effects of proanthocyanidin on radiation-induced injury of AHH-1 and HIEC cells,and to explore the possible molecular mechanism. Methods： CCK-8 assay was applied to examine the survival of AHH-1,HIEC cells after 7-ray irradiation with or without proanthocyanidin pretreatment. Annexin-V/PI staining and flow cytometry analysis were used to evaluate the apoptosis and cell cycle of AHH 1 and HIEC cells. The expression of Bcl-2 protein was analyzed by Western blotting assay. Results： Pretreatment with proanthocyanidin significantly increased the cell survival rate after radiation（P〈0.01）. The apoptosis rates of cells in the proanthocyanidin＋ radiation group were （11.78 % at 4 Gy[AHH- 1] and 5.32% at 8 Gy[HIEC],respectively） greatly lower than those of the single radiation group （26.38% at 4 Gy[AHH-1] and 12.45% at 8 Gy[HIEC],respectively,P〈0.01）. Moreover,the inhibition of Bcl-2 protein expression in AHH-1 ,HIEC cells was attenuated in the proanthocyanidin ＋ radiation group. Conclusion： Proanthocyanidin has satisfactory protective effect on radiation-induced cellular injury; the mechanism may be related to the attenuation of Bcl-2 protein inhibition.