动脉粥样硬化斑块和血管壁的荧光光谱学实验研究

Spectroscopy Study of Atherosclerotic Plaque and Vessel Wall

为区分动脉粥样硬化斑块和血管壁,以给激光血管成形术的实时监控提供依据,我们研究了动脉粥样硬化斑块和血管壁的激光诱导荧光光谱。选用日本雄性大耳白兔15只,以高脂饲料喂养3个月,取主动脉弓作纵行切开,分别采用380nm紫外激光和532nm绿激光作为激发光源诱导动脉粥样硬化斑块和血管壁的荧光光谱。结果显示:380nm紫外激光诱导的动脉粥样硬化斑块和血管壁的荧光光谱在416nm处均有明显特征峰,但前者的强度显著高于后者;532nm绿激光诱导的动脉粥样硬化斑块和血管壁的荧光光谱均在532nm和800nm处有明显特征峰,但强度无明显差别。说明紫外激光诱导的荧光光谱可以有效区分动脉粥样硬化斑块和血管壁,而532nm绿激光诱导的荧光光谱不能有效区分动脉粥样硬化斑块和血管壁。

To differentiate atherosclerotic plaque and vessel wall by laser-induced fluorescence spectroscopy （LIFS）, providing a real-time monitoring for laser angioplasty. Fiftecn male Japanesc white rabbits, feeding three months with high-fat feed. Get aortic arch and do portrait incision, using 380nm UV laser and 532nm green laser as light source to induce fluorescence spectroscopy of atherosclerotic plaque and vessel wall respectively. The results showed that： 380nm UV laser-induced fluorescence spectra of atherosclerotic plaque and vessel wall both have characteristic peaks at wavelength of 416nm, but the intensity of the former is significantly higher than the latter; 532nm green laser-induced fluorescence spectra both have characteristic peaks at wavelength of 532nm and 800nm, but no significant difference in intensity. Therefore, 380nm UV laser-induced fluorescence spectroscopy can effectively differentiate atherosclerotic plaque and vessel wall. while 532nm green laser-induced fluorescence spectra can not effectively differentiate them.