NBD多肽对大鼠急性坏死性胰腺炎的影响

Effects of NF-κB essential modifier binding domain peptides on rats with acute necrotizing pancreatitis

目的通过NF-κB基本修饰结合域（NF-κB essential modifier binding domain，NBD）多肽预处理，观察其对大鼠急性坏死性胰腺炎（ANP）的影响。方法逆行胰胆管内注射5％牛磺胆酸钠制备大鼠ANP模型。术前30min腹腔注射NBD预处理。术后6、12h处死大鼠，观察胰腺组织病理，检测胰腺组织NF—κBp65蛋白表达和血清淀粉酶、IL-6、TNF-α水平的变化。结果ANP大鼠6h时血清TNF—α和IL-6水平、胰腺病理分值、NF-κB p65蛋白阳性表达率分别为（76±6．7）ng／L、（387±67）ng／L、8．71±0．45、（49．26±2．15）％；多肽预处理后血清TNF-α和IL-6水平、胰腺病理分值、NF-κB p65蛋白阳性表达率分别为（48±4．5）ng／L、（295±48）ng／L、6．76±0．26、（37．46±1．68）％，两组相差均非常显著（P〈0．05）。结论NBD多肽预处理可抑制ANP大鼠胰腺组织NF-κB的过度活化，下调炎症因子TNF-α、IL-6的表达，减轻胰腺损伤。

Objective To investigate the effect of pretreatment of nuclear factor-kappa B （NF-κB） essential modifier binding domain （NBD） on acute necrotizing pancreatitis （ANP）. Methods 5% sodium taurocholate was retrograde injected into the biliopancreatic ducts to induce the ANP model. NBD was injected intraperitoneally into the rats 30 minutes before the operation. The rats were sacrificed 6 or 12 h after operation, and the pancreatic histopathology scores, NF-κB P65 protein expression, serum amylase and serum TNF-alpha, IL-6 were detected. Results The serum level of TNF-alpha, IL-6, pancreatic histopathology scores, NF-κB P65 protein positive expression rate were （76 ± 6.7） ng/L, （387 ± 67） ng/L, 8, 71 ± 0.45 and （49.26 ± 2.15 ） %, respectively, in ANP group; after NBD pretreatment, the corresponding values were （48 ± 4.5 ） rig/L, （295 ± 48 ） ng/L, 6.76 ± 0.26 and （ 37.46 ± 1.68 ） %, respectively ; and the difference was statistically significant （P 〈 O. 05）. Conclusions The NBD peptides could inhibit the over-activation of NF- kappa B, down-regulate the expression of IL-6 and TNF-alpha, alleviate the inflammation of ANP.