nNOS、VEGF和Bcl-2蛋白在人胚胎发育中脊髓前角的表达及意义

Expression and significance of nNOS,VEGF and Bcl-2 proteins in the developing anterior horn of human embryonic spinal cord

目的探讨神经元型一氧化氮合酶（nNOS）、血管内皮生长因子（VEGF）和B细胞淋巴瘤/白血病-2（Bcl-2）蛋白在人胚胎发育早期脊髓前角中的分布规律及其表达意义。方法应用免疫组织化学法检测第2、3、4月龄段,人胚胎脊髓前角中nNOS、VEGF和Bcl-2蛋白的表达。结果在第2-4个月龄段,nNOS和Bcl-2蛋白在人胚胎脊髓前角组织细胞中由弱阳性表达逐渐变为阳性表达。在第2个月龄段,VEGF蛋白在人胚胎脊髓前角组织中均呈阴性表达;第3-4个月龄段,VEGF蛋白在人胚胎脊髓前角组织细胞中由弱阳性表达逐渐变为阳性表达。结论nNOS、Bcl-2和VEGF蛋白与人胚胎脊髓前角神经元的生长发育关系密切。

Objective To explore the rule of distribution and expression significance of neuronal nitric oxide synthase （nNOS）, vascular endothelial growth factor （VEGF） and B-cell lymphoma/leukemia-2（Bcl-2）proteins in the developing spinal cord anterior horn of human embryos. Methods In the fourth month of gestation, the expression productions of nNOS, VEGF and Bel-2 proteins were investigated in the anterior horn of human embryonic spinal cord using the immunohistochemical methods. Results The intensities of the immunohistochemical signals of both nNOS and Bcl-2 proteins were progressively enhanced from the second to the fourth month of gestation. There was negative immunohistochemical signals of VEGF in the anterior horn of human embryonic spinal cord in the second month of gestation. At the third to fourth month of gestation, the expression of VEGF protein was from weak to strong in the anterior horn of human embryonic spinal cord. Conclusion nNOS, VEGF and Bcl-2 proteins are related to the growth and development of neurons in the spinal cord anterior horn of human embryos.