三种改良的阮森吡啶-硝酸银法显示大鼠脊髓组织的比较

Comparision of three modified Ranson’s methods on displaying rats’ spinal cord tissue

目的对3种改良的阮森吡啶-硝酸银法（Ranson）在显示大鼠脊髓组织中的应用进行比较。方法对照组采用传统的阮森吡啶-硝酸银法,即取大鼠的新鲜脊髓组织,在酒精中加纯氨水固定,经纯吡啶、硝酸银、还原液染色,常规石蜡切片并观察。实验组采用改良的阮森吡啶-硝酸银法,即分别采用NaOH溶液、Na2CO3溶液和95%酒精替换在对照组中使用的纯氨水,其他染色步骤同对照组。结果实验组的脊髓组织切片分别经3种改良的阮森吡啶-硝酸银法染色,神经元的胞体和突起均呈黄棕色,神经纤维呈棕褐色,切片染色结果与对照组比较无明显差异,尤以酒精替换组的染色效果为最佳。结论以改良的阮森吡啶-硝酸银法显示大鼠脊髓组织中神经元及神经纤维染色效果良好,方法更加简便易行,且避免了氨水的刺激气味,有利于改善教学和科研的局部工作环境。

Objective The aim of this study was to compare effects of three modified Ranson＇ s methods on the staining of spinal cord of rats. Methods In the control group, following fixed with alcohol and ammonia, the tissue of spinal cord was embedded with wax, sectioned and stained with traditional Ranson＇ s method, then, observed under the light microscope. For experimental groups, the tissue of spinal cord was treated with three modified methods namely, replaced the ammonia with sodium hydroxide, sodium carbonate or 95 % alcohol respectively, other staining processes were as same as those in the traditional Ranson＇ s staining method. Results On slices of the experimental group, the soma and neuron filaments were showed with color of yellow- brown, and those nerve fibers were stained with deep brown. Compared with the control group, no significant difference was found in three experimental groups. However, the best staining result was found in the group which the ammonia was replaced with 95 % alcohol. Conclusion Three modified Ranson＇s staining methods showed a satisfying staining on the neuron soma and fibers.