IL-1β对醋氨酚诱导的离体小鼠肝细胞损伤的保护作用

PROTECTIVE EFFECT OF INTERLEUKIN1β ON ACETAMINOPHENINDUCED INJURY IN MOUSE HEPATOCYTES

目的：在原代培养的小鼠肝细胞上观察白细胞介素１β（ＩＬ１β）对醋氨酚诱导的细胞损伤的影响及其与一氧化氮、环磷酸鸟苷（ｃＧＭＰ）的关系。方法：以两步灌流法分离小鼠肝细胞，进行原代培养；以放射免疫法测定细胞内ｃＧＭＰ和环磷酸腺苷（ｃＡＭＰ）含量。结果：ＩＬ１β可减轻醋氨酚诱导的转氨酶漏出，增加肝细胞内ｃＧＭＰ含量，一氧化氮合酶阻断剂ＬＮＭＭＡ可完全阻断ＩＬ１β的抗损伤作用。结论：ＩＬ１β对醋氨酚诱导的肝细胞损伤具有直接的保护作用，这一作用可能是通过一氧化氮、ｃＧＭＰ介导的。

Objective: To investigate the effect of interleukin1β on acetaminopheninduced cell injury and its relation to nitric oxide and cGMP in primary culture of mouse hepatocytes. Methods: Mouse hepatocytes were isolated by two-step perfusion and cultured primarily. cGMP and cAMP contents were measured by radioimmunoassay. Results: Interleukin1β could reduce acetaminopheninduced leakage of aminotransferases and increase the content of cGMP in hepatocytes. LNMMA, an inhibitor of nitric oxide synthase, could completely abolish the antiinjury effect of interleukin1β. Conclusion: Interleukin1β had direct protective effect on acetaminopheninduced injury in hepatocytes and this effect might be mediated by nitric oxide and cGMP.