猪肚木叶中多酚含量测定及抗氧化活性研究

Study on determination of total polyphenolic content and antioxidant activity of the leaves from Canthium horridum Bl.

采用紫外分光光度法,以焦性没食子酸为标准品,在波长540nm处,对猪肚木叶提取物中总多酚类化合物含量进行了测定,并用流动注射化学发光法研究了猪肚木叶的抗氧化性能。实验结果表明,猪肚木叶中总多酚含量达0.4879%,标准曲线的回归方程Y=16.76X-0.0948,r=0.9997,RSD=0.745%;猪肚木叶具有较强的自由基清除能力,其IC50为0.35mg/mL。

Method of UV-spectrophotometry was used at the wavelength of 540nm to quantify total polyphenol of the leaves from Canthium horridum BI. ,taking pyrogallic acid as standard.This thesis also determined antioxidation of the leaves of Canthium horridum BI.By flow injection chemiluminescence method using luminol-H2O2 system.The results showed as follows ： the content of polyphenols was 0.4879%.The regression equation with a good linearity （r =0.9997） between the absorbance and concentration was found. RSD was 0.745%. The leaves of Canthium horridum BLhad strong antioxidant activity.The IC50 values were found to be 0.35mg/mL.