摘要
以负载型结晶尿素为载体，进行了不同脂肪酸乙酯的吸附柱分离，考查了负载型尿素柱分离α-亚麻酸乙酯过程中原料种类、固定相尿素硅胶比例及硅胶粒度等的影响。结果表明：固定相中尿素与硅胶最佳质量比为2：3，以紫苏油乙酯为原料，60-80目硅胶为柱层层析载体，无水甲醇为负载尿素的结晶溶剂，石油醚为流动相．层析柱规格为2cm×60cm，进样量为7mL，洗脱液流速范围为1～1．5ml/min，可得到纯度85．55％的α-亚麻酸乙酯，收率81％。
The selective adsorption of fatty acid ethyl ester with different saturation on crystal-urea was studied. Based on this selective adsorption, the feasibility of separation of ethyl α-linolenate by urea column chromatography was investigated, and the factors influencing ethyl α-linolenate separation by urea column chromatography were also studied.The results showed that when the ratio of urea to silica gel in the stationary phase was 2：3 （w/w） ,the raw material was ethyl ester of perilla oil, the carriers was silica gel （60-80 mesh）, the load solvent of urea was methanol,the mobile phase was petroleum ether,the column chromatography was 2cm × 60cm,the loading amount was 7mL and the flow rate of eluate range was 1- 1.5mL/min, the purity of ethyl α-linolenate can be up to 85.55% ,and the yield can be up to 81%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第6期292-294,共3页
Science and Technology of Food Industry
关键词
Α-亚麻酸乙酯
分离
提纯
尿素
柱层析
ethyl α-linolenate
separation
purification
urea
column chromatography
