摘要
采用等吸收点双波长紫外分光光度法,对以肉桂酸为底物,微生物转化合成苯乙酮转化液中的肉桂酸和苯乙酮,并进行直接定量分析。选择菌体发酵液作参比,检测波长分别为249nm和298nm。苯乙酮的检出限为0.22μg·mL-1(S/N=3),肉桂酸的检出限为0.24μg·mL-1(S/N=3)。两者测定结果的平均相对标准偏差分别为0.13%和0.24%;平均回收率为104.17%和97.92%。
A method for determination directly of acetophenone and cinnamic acid in the bioconversion broth of acetophenone from cinnamic acid by dual-wavelength UV spectrophetometry was developed.The fermentation broth was used as reference solution and the wave-lengths selected were 249nm and 298nm.The results showed that the detection limits of acetophenone and cinnamic acid were 0.22μg.mL^-1 and 0.24μg.mL^-1 ( S/N = 3).Both determination results of the relative standard deviation were 0.13% and 0.24%, and the average recoveries were 104.17 % and 97.92%, respectively.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第6期336-337,359,共3页
Science and Technology of Food Industry
基金
广西科学基金资助项目(桂科回0448002)
广西应用基础研究专项(桂科基0832002)
关键词
双波长紫外分光光度法
生物转化液
苯乙酮
肉桂酸
dual-wavelength ultraviolet spectrometry
bioconversion broth
acetophenone
cinnamic acid