人工肠炎鼠硬化性胆管炎的研究

Experimental Study on Primary Sclerosing Cholagitis in Rats

目的：进一步探讨原发性硬化性胆管炎的发病机理。方法：对人工肠炎的大鼠直肠灌注大肠杆菌趋化性多肽（ｃｐ－ｆＭＬＴ），以造成原发性硬化性胆管炎（ＰＳＣ）的动物模型。４ｄ后对肝组织进行光镜、免疫组化及电镜观察。结果：大鼠肝动、静脉及胆管有明显的炎症表现，肝细胞出现坏死；浸润的细胞主要是淋巴细胞，且多集中于小胆管周围，可与胆管的上皮细胞结合，少数胆管上皮细胞萎缩或崩解。结论：ｃｐ－ｆＭＬＴ可通过细胞介导机理诱导产生小胆管炎，在小胆管ＰＳＣ的发病机理中起重要作用。

Objective:We studied the pathogenesis of the primary sclerosing cholangitis (PSC) since inflammatory bowel diseases (IBD) frequently accompany PSC. Methods:We observed bile duct changes in rats with colitis which had been made by rectal infusion of one of the chemotactic peptide produced by E. Coli, N formyl L methonine L leucine L tyrosine (cp fMLT).Light, electrony microscopy, and immunohistochemical methods had been used to evaluate changes of bile duct in this paper.Results:[WT5”BZ]Our results showed that the infiltrating leukocytes in the portal tract were mostly lymphocytes, which,especially the small ones, densely infiltrated around the bile ducts. These infiltrated leukocytes may combine with the epithelium, and resulted in atrophy or apoptosis of epithelial cells. Conclusions:[WT5”BZ] cp fMLT may mediate pathogenesis of small duct inflammation via a leukocytic mechanism.