对硝基苯酚降解菌Pseudomonas sp.HY1的分离与活性分析

Biodegradation of p-Nitrophenol by Pseudomonas sp.HY1

通过驯化富集,从受污染的土壤中分离出一株降解对硝基苯酚(p-nitrophenol,PNP)的细菌.16S rDNA序列分析鉴定该菌为恶臭假单胞菌Pseudomonas sp.HY1.在有氧,pH7和30℃条件下,该菌能利用PNP为碳源和能源生长并将中等浓度(100 mg/L)的PNP快速彻底的降解,高浓度(300 mg/L)PNP条件下未检测到菌的生长和降解活性.该菌在15～40℃和pH值5～10的条件下具有降解PNP活性,其中碱性条件(pH 8～10)和30℃时活性最高.

A bacterial strain （HY1）, capable of using p-nitrophenol （PNP） as a sole source of carbon and energy, was isolated from the pesticide-polluted soil. Analysis of the 16s rDNA gene sequence suggested that Y1 had a close relationship to Pseudomonas putida and was designated as Pseudomonas sp. HY1. Degradation studies showed that PNP was degraded quickly under aerobic condition, complete depletion of 50 mg/L and 100 mg/L of PNP was achieved in 10 h and 24 h respectively by HY1 at pH of 7 and temperature of 30 ℃. No growth of HY1 and PNP degradation by HY1 were detected when PNP concentration reached 300 mg/L in the minimal salt media. The greatest growth of HY1 was observed at temperature of 30 ℃ and alkaline pH （pH 8-10）.