血府逐瘀汤对动脉粥样硬化家兔血清非对称性二甲基精氨酸水平的影响

Effects of Xuefu Zhuyu decoction on serum asymmetric dimethylarginine in atherosclerosis rabbits

目的:观察血府逐瘀汤对动脉粥样硬化家兔血清非对称性二甲基精氨酸(asymmetric dimethylarginine,ADMA)的影响,并探讨血府逐瘀汤抗动脉粥样硬化的机制。方法:24只雄性日本大耳家兔,随机分为3组(每组8只):正常组喂饲普通饲料;模型组喂饲高胆固醇高脂饲料100 g;血府逐瘀汤组在造模同时,灌胃给血府逐瘀汤7.10 g.kg-1(含生药),每天1次。喂养8周后,测定家兔血清甘油三脂(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、血清ADMA和血清一氧化氮(NO)的浓度;取兔主动脉制成切片在光学显微镜下观察病理改变,并应用Optimas显微图像分析系统,测主动脉粥样硬化斑块灰度及斑块面积。结果:模型组家兔主动脉粥样硬化斑块灰度(198.91±5.08)及斑块面积(4.80±0.83)mm2,均显著高于血府逐瘀汤组家兔斑块灰度(175.56±12.30)及斑块面积(1.96±0.48)mm2(P<0.01),正常组家兔主动脉无动脉粥样硬化斑块形成。模型组家兔血清TC(23.42±7.80)mmol.L-1,TG(2.37±0.42)mmol.L-1,LDL(17.24±8.29)mmol.L-1明显高于血府逐瘀汤组TC(15.63±4.12)mmol.L-1,TG(1.43±0.34)mmol.L-1,LDL(7.64±2.36)mmol.L-1(均P<0.01)。血府逐瘀汤组家兔血清TC,LDL明显高于正常组TC(P<0.01),而血府逐瘀汤组家兔血清TG与正常组TG无明显差异。模型组家兔血清ADMA水平(1.57±0.17)μmol.L-1明显高于血府逐瘀汤组(1.01±0.13)μmol.L-1(P<0.01),而NO水平(8.76±2.42)μmol.L-1明显低于血府逐瘀汤组(15.90±2.38)μmol.L-1(P<0.01)。血府逐瘀汤组家兔血清ADMA及NO水平与正常组ADMA及NO水平无明显差别。家兔血清ADMA与NO水平呈负相关关系(r=-0.773,P<0.01)。结论:血府逐瘀汤可以降低动脉粥样硬化家兔血清ADMA水平,降低血清ADMA水平可能是其抗动脉粥样硬化的作用机制之一。

Objective： To observe the effect of Xuefu Zhuyu decoction on serum asymmetric dimethylarginine （ADMA） level of the atherosclerosis rabbits. Method： Twenty-four male rabbits were randomly divided into three groups ： control group （n = 8） was fed with normal diet ; the model group （ n = 8 ） was fed with high fat/high cholesterol diet ; and Xuefu Zhuyu decoction group （ n = 8） was fed with high fat/high cholesterol diet and intragastric administrated with Xuefu Zhuyu decoction 7. 10 g.kg^-1 . d^-1. Concentrations of serum total cholesterol （TC）, total triglyceride （TG）, low density lipoprotein （LDL）, serum ADMA and serum nitric oxide （NO） were measured after eight weeks. Rabbits＇aorta were made into the pathological section to observe the pathology changes degree and atherosclerotic plaqu mean gray value and plaque area were analyzed by OPTIMAS image analysis system. Result： （1）Compared with the aortic atherosclerotic plaqu mean gray value of Xuefu Zhuyu decoction group 175.56 ± 12. 30, those of the model group 198.91 ±5.08 were significant higher （P 〈 0.01 ）. Significant difference were found between the the aortic atherosclerotic plaque area of the model group （4. 80 ± 0. 83 ） mm^2 and Xuefu Zhuyu decoction group （ 1.96 ± 0.48 ）mm^2 （ P 〈 0.01 ）. There were no atherosclerotic plaque in the control group rabbits＇aorta. （2）The serum TC （23.42 ±7. 80） mmol.L^-1 , TG （2.37±0.42） mmol.L^-1 and LDL （ 17.24 ± 8.29） mmol.L^ - 1 of the model group were higher than those of Xuefu Zhuyu decoction group （ P 〈 0. 01 ） ; Compared with control group, the serum TC （ 15.63 ± 4. 12 ） mmol.L^-1 and LDL （7. 64 ± 2. 36 ）mmol .L^- 1 of the Xuefu Zhuyu decoction group increased significantly, while there were no significant changes between serum TG of control group （1.11 ±0. 26） mmol.L^-1 and serum TG of Xuefu Zhuyn decoction group （ 1.43 ± 0. 34 ） mmol.L^-1. The serum ADMA of the model group （ 1.57 ± 0. 17 ） μmol. L^-1 were higher than those of Xuefu Zhuyu decoction group （ 1.01 ± 0. 13 ） μ mol .L^ - 1 （ p 〈 0. 01 ）, while the serum NO of the model group （8.76± 2.42）μmol.L^-1 were significantly lower than those of Xuefu Zhuyu decoction group （ 15.90 ± 2. 38 ）μmol.L^-1 （P 〈0. 01 ）. There were no significant difference between the serum ADMA of the control group （0. 93 ± 0. 11 ） μmol.L^-1 and Xuefu Zhuyu decoction group. Compared with Xuefu Zhuyu decoction group, the serum NO of the control group （17. 88 ± 3.34） μmol.L^-1 have no significant difference. （3）The serum ADMA of all rabbits negatively correlated to the serum NO （ r = - 0. 773 ）, （ P 〈 0. 01 ）. Conclusion ： Xuefu Zhuyu decoction can decrease the serum ADMA level in atherosclerosis rabbits. The effect of Xuefu Zhuyu decoction on anti-atherosclcrosis may be related to the decreased serum ADMA level