人参皂苷Re在大鼠体内的代谢研究

Metabolic study of ginsenoside Re in rats

目的:研究人参皂苷Re在大鼠体内的代谢情况并了解其代谢产物。方法:将6只SD大鼠按100 mg.kg-1灌胃给予人参皂苷Re,收集给药后12,24,36,48,60,72 h粪便,预处理后采用HPLC-ESI-MS/MS方法,以人参皂苷Re的微生物转化产物为对照品,检测大鼠体内的代谢产物。结果:在大鼠体内共检测到6个代谢产物,并利用对照品鉴定了它们的结构分别为:20(S)-人参皂苷Rg2,20(S)-人参皂苷Rh1,20(R)-人参皂苷Rh1,人参皂苷F1,3-羰基人参皂苷Rh1和原人参三醇。结论:人参皂苷Re在大鼠体内的代谢产物较多,且与微生物转化产物具有很强的相似性。

Objective： To systemically study the metabolism of ginsenoside Re in rats. Method： Six SD rats were used divided into 3 groups. After oral administration of ginsenoside Re at a dosage of 100 mg .kg^-1, feces were collected at 12, 24, 36, 48, and 60 h. The microbial transformation metabolites of ginsenoside Re were used as standard references. HPLC-ESI-MS/MS analysis was used in the metabolism studies. Result： Six metabolites of ginsenoside Re were detected in rat feces by HPLC-ESI-MS/MS analysis. Their structures were identified as 20 （S）-ginsenoside Rg2, 20 （S）-ginsenoside Rh1 , 20 （R）-ginsenoside Rh1 , ginsenoside F1 , 3-oxo- ginsenoside Rh1 and protopanaxatriol. Conclusion： Ginsenoside Re has the same metabolic pathway with microorganisms, to some extent, justified the use of microbial models for mammalian metabolism studies.