CXCR1/2在剪应力诱导血管内皮细胞迁移中的作用

The Role of CXCR1/2 in Shear Stress-induced Endothelial Cell Migration

CXCR1/2是调节血管内皮细胞生理活动的重要受体。为研究CXCR1/2在剪应力诱导血管内皮细胞迁移中的作用,我们检测了剪应力作用下内皮细胞CXCR1和CXCR2表达变化;并以anti-IL8RA和anti-IL8RB拮抗CXCR1和CXCR2,由流室系统提供不同大小的剪应力,对比对照组与受体拮抗组内皮细胞在划痕实验中的迁移情况。结果表明,CXCR1和CXCR2被anti-IL8RA和anti-IL8RB拮抗后,内皮细胞的迁移也被有效抑制。其中,anti-IL8RA的抑制作用强于anti-IL8RB(P<0.05);在同时加入两种受体拮抗剂的情况下,内皮细胞迁移受到进一步抑制。以上结果提示,CXCR1和CXCR2是调节剪应力诱导内皮细胞迁移的重要因素,而以CXCR1作用更为明显。

CXCR1 and CXCR2 are important receptors in regulating vascular endothelial cell activities. In order to elucidate the role of CXCR1/2 in shear stress-induced endothelial cell migration, we have investigated the expression levels of CXCR1 and CXCR2 in the endothelial cells exposed to shear stress. In the experiment, anti-IL8RA and anti-IL8RB were used to antagonize CXCR1 and CXCR2. Different shear stresses were generated in a flow chamber; scratch test was carried out to compare endothelial cell migration in the control group and the receptor-antagonized groups. The results indicated that the migration of endothelial cells was restrained effectively after CXCR1 and CX- CR2 were antagonized by anti-IL8RA and anti-IL8RB. And anti-ILSRA showed a stronger inhibitive effect than did anti-IL8RB （P〈0. 05）. In the group with both receptor antagonisms, the migration was further inhibited. These results suggest that both CXCR1 and CXCR2 are important factors in mediating the migration of endothelial cells induced by shear stress, and CXCR1 fulfills a more important role.