二血管阻断加低血压法建立大鼠全脑缺血再灌注模型的实验研究

Experimental study of establishing the global cerebral ischemia/reperfusion model by two vessel occluaion plus hypotenaion in rats

目的通过二血管阻断加低血压法建立大鼠全脑缺血再灌注模型，探讨全脑缺血再灌注大鼠大脑组织结构变化，为脑复苏中的进一步神经保护作用研究提供条件。方法30只SD大鼠采用二血管阻断加低血压法制作大鼠全脑缺血再灌注模型；实验结束后，计算成功例数和死亡例数。开颅取大脑组织进行形态学观察。结果30只大鼠中，26只造模成功，成功率86．67％。全脑缺血再灌注大鼠大脑出现大量神经元坏死、胞浆浓缩、核固缩现象。结论二血管阻断加低血压法成模效果稳定，死亡率较低。能较好地建立大鼠全脑缺血再灌注模型，可为脑复苏的的深入研究提供良好实验基础。

Objective Through two vessel occlusion add hypotension to establish the global cerebral ischemia/reperfusion model, discusses the global cerebral structure change in rats.Methods Global cerebral ischemia-reperfusion model was established by two-vessel occlusion plus hypotension in 30 SD rats. Successful cases and death cases were calculated and cerebral morphological changes were observed, Results Of 30 rats, 26 （86. 67%） were successful, and morphological observation showed obvious neurons lesions including neurons necrosis, concentrated cytoplasma and pyknosis. Conclusions The method of two vessel occlusion plus hypotension could establish global cerebral ischemia/reperfusion model in rat. It was stability, typical and lower mortality.