光合细菌利用葡萄糖产氢过程中菌体衰亡性研究

STUDY ON CELL GROWTH DECLINE OF PHOTOSYNTHETIC BACTERIA IN HYDROGEN PRODUCTION UTILIZING GLUCOSE

研究了葡萄糖作为产氢基质在产氢过程中对光合细菌的生长活性及产氢的影响,分析了产氢过程中菌体生长活性下降发生衰亡的原因,并就抑制菌体发生衰亡的相关措施进行了初步探讨。研究发现:光合细菌利用葡萄糖产氢过程中细胞生长活性下降迅速,葡萄糖会抑制菌体的生长,促使细胞衰亡。产氢过程中批次添加葡萄糖虽然可以在一定程度上抑制菌体的衰亡,但对产氢并没有促进作用,产氢速率低,产氢总量只达到对照组的87%。产氢过程中外源调节菌液pH值对菌体的生长及产氢有显著促进作用,细胞生长达到对照组的1.46倍,产氢总量达到对照组的1.36倍。

The influence of glucose as hydrogen production substrate on the growth activity of photosynthetic bacteria and the capacity of hydrogen production was studied, the reasons of decreasing cell growth activity inducing cell growth decline were analyzed, and the relative measures which inhibited cell growth decline were discussed preliminarily in this pa- per. The results indicated that cell growth activity was decreased quickly and the glucose could inhibit the cell growth and induce cell growth decline in the process of hydrogen production utilizing glucose. Adding glucose in batch was beneficial to inhibit cell growth decline to a certain extent, but had no promotion on hydrogen production, hydrogen production rate was low and the total yield of hydrogen production only reached 87 % of the control group. The exogenous regulation of pH had significant promotion on cell growth and hydrogen production, and cell growth reached 1.46 times and the total yield of hydrogen production reached 1.36 times of the control group.