膀胱移行细胞癌组织中B7-H1和PTEN的表达及意义

Expression and significance of B7-H1 and PTEN in bladder transitional cell carcinoma

目的探讨共刺激分子B7-H1和PTEN在膀胱移行细胞癌组织中的表达变化及意义。方法采用免疫组化SP法检测50例膀胱移行细胞癌及10例正常膀胱组织中的B7-H1和PTEN,分析其与膀胱移行细胞癌临床病理参数的关系及两者的相关性。结果正常膀胱组织中B7-H1不表达,膀胱移行细胞癌组织中B7-H1的阳性表达率为72%,且B7-H1的表达与肿瘤的病理分级、临床分期及复发密切相关(P<0.05);正常膀胱组织中PTEN呈阳性表达,膀胱移行细胞癌中PTEN蛋白阳性表达率为58%,随着肿瘤病理分级、临床分期的增加PTEN表达显著降低(P<0.05)。B7-H1与PTEN的表达呈明显负相关(r=-0.44,P<0.01)。结论膀胱移行细胞癌中B7-H1的高表达和PTEN的突变或缺失与肿瘤的发生发展和免疫逃逸密切相关。

Objective To investigate the expression and significance of B7-H1 and PTEN in bladder transitional cell carcinoma. Methods The expression of B7-H1 and PTEN in 50 eases of bladder transitional cell carcinoma was detected by immunohistochemieal SP method and the relationship between the expression and clinical pathological parameters of bladder transitional cell carcinoma was also analyzed. Result In normal bladder t＇issue, B7-H1 was not detected. But in bladdertransitional eel/carcinoma, the positive rate of B7-H1 was 72% and B7-H1 expression was strongly associated with pathological grade, clinical stage and recurrence （ P 〈 0.05 ）. In normal bladder tissue, PTEN was all detected. But in bladder transitional cell carcinoma, the positive rate of PTEN was only 58% and frI＇EN expression was also associated with pathological grade and clinical stage （ P 〈 0.05 ）. And BT-H1 expression had a negative correlation with PTEN （ r = - 0.44, P 〈 O. 01 ）. Conclusions The high expression of B7-H1 and the loss of [rI＇EN are strongly associated with neoplastic proggression and may play an important role in immune escape of bladder transitional cell carcinoma.