survivin启动子的真核表达载体构建

Construction of Eukaryotic Expression Vector Drived by Survivin Gene Promoter

目的构建以survivin启动子驱动增强绿色荧光蛋白(EGFP)表达的真核表达载体pEGFP-C1/Surv。方法用PCR扩增survivin启动子,构建其真核表达载体并检测survivin启动子的活性。结果PCR成功扩增出survivin启动子,其真核表达载体构建成功。结论survivin启动子能驱动EGFP的表达,证明其具有活性。

Objective To construct pEGFP - C1/Surv eukaryotic expression vector in which the EGFP was drived by survivin gene promoter. Methods We amplified the survivin gene promoter by PCR and constructed pEGFP -C1/Surv plasmid in which the EGFP was drived by survivin gene promoter, and detected the activity of survivin gene promoter. Results The survivin gene promoter was amplified successfully; the pEGFP- C1/Surv eukaryotic expression vector was constructed successfully. Conclusion The survivin gene promoter could drive the expression of EGFP,so its activity was verified.