金鱼草基因转化中卡那霉素筛选压力的确定

Confirmation of Selective Concentration of Kanamycin in Genetic Transformation for Antirrhinura majus L.

在以抗生素为筛选标记的基因转化中,确定适宜的筛选压力是提高基因转化率的前提。本研究以卡那霉素为筛选标记,在10、20、30、50和100 mg/L的筛选压力下,根据金鱼草下胚轴外植体的生长表现、愈伤组织和芽的分化率确定适宜的筛选浓度。结果表明,适宜卡那霉素的筛选浓度为50 mg/L。以此浓度为筛选压力进行遗传转化所获得的部分再生苗经Southern blot鉴定,均为转化株。

In order to confirm the optimum selective concentration of antibiotic in genetic transformation, kanamycin was selected as a case in this paper. It had been found that the optimum selective concentration of kanamycin was 50 mg/L according to the selective effects, such as the growth and the difference of explants of Antirrhinura majus L. on the medium with different concentrations of 10, 20, 30, 50 and 100 mg/L, respectively. Some shoots of transformation selected by 50 mg/L kanamycin were confirmed by Southern blot.