丁香酚乳液和醇溶液对鲤鱼麻醉作用的对比研究

Contrast Study of Anesthetic Effect of Emulsified Eugenol and Alcoholic Eugenol on Cyprinus carpio Linnaeus

以吐温80、司盘80、无水乙醇和水为原料制成丁香酚乳液,对鲤鱼进行麻醉,对比测定丁香酚乳液和丁香酚醇溶液对鲤鱼的麻醉开始时间、麻醉持续时间和LD50。结果表明:在相同浓度下,丁香酚乳液对鲤鱼的麻醉开始时间比丁香酚醇溶液慢,作用较平缓,但麻醉持续时间长5倍。丁香酚乳液的半数致死量比丁香酚醇溶液大2.15倍。因此,丁香酚乳液对鲤鱼的麻醉作用较缓和,麻醉维持时间长,安全性更高,更适合用于生产实践。

To test the anesthetic effect of emulsified eugenol to Cyprinus carpio Linnaeus, eugenol was emulsified with Tween 80, Span 80, ethanol and water. Then the start time, time length of anesthesia and LD50 of emulsified eugenol to Cyprinus carpio Linnaeus was de- termined contrastively with alcoholic eugenol. Results showed that the start time of anesthesia of emulsified eugenol was longer than these of alcoholic eugenol under the same concentration of eugenol. The time length and LD50 of anesthesia of emulsified eugenol were five times longer and 2.15 times large respectively than that of alcoholic eugenol. So, the anesthetic effect of emulsified eugenol on Cyprinus carpio Linnaeus was slower, gentler, longer and safer than alcoholic eugenol. It was more proper to be applied for fish farming than alcoholic eugenol as a fish anesthetic agent.