姜黄素对HSC PDGF-BB、PDGFRβ及ERK_1表达的影响被引量：4

Study on Effects of Curcumin on Expressions of PDGF-BB,PDGFRβ and ERK_1 of HSC

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摘要目的:探讨姜黄素对大鼠HSC-T6表达PDGF-BB、PDGFRβ、ERK1的影响。方法:采用MTT法观察不同质量浓度姜黄素对HSC细胞增殖的影响,并计算其抑制率,确定姜黄素最佳等比浓度,设对照组、TGF-β1刺激组和姜黄素各质量浓度干预组。采用免疫组化、聚合酶链式反应(RT-PCR)半定量法探讨姜黄素对HSC-T6PDGF-BB、PDGFRβ、ERK1表达的影响。结果:正常培养的HSC-T6均有PDGF-BB、PDGFRβ、ERK1表达,经姜黄素不同质量浓度干预后,PDGF-BB、PDGFRβ、ERK1表达降低,且有一定的剂量依赖性。结论:姜黄素能抑制HSC-T6PDGF-BB、PDGFRβ、ERK1的表达,这可能是其抗肝纤维化的作用机理。 Objective：To observe the effects of curcumin on expression of PDGF-BB,PDGFRβ and ERK1 of rat Hepatic stellate cell（HSC-T6）. Methods：The cultured HSC were divided into control group,each curcumin treating group. The influence of cureumin on HSC proliferation was detected by MTT assay. The protein levels of PDGF-BB , PDGFRβ and ERK1 was detected by immunhistochemical examination;The influence of curcumin on expression of PDGF-BB, PDGFRβ and ERK1 mRNA was detected by RT-PCR. Resuhs：Immunohistiochemical results showed that normal cultured HSC-T6 cell expressed PDGF-BB, PDGFRβ and ERK1 remarkably. After the intervention of different concentration of curcumin,the masculine degrees of PDGF-BB,PDGFRβ and ERK1 and the masculine cell population decreased obviously. The RT-PCR results indicated that the expressions of PDGF-BB, PDGFRβ and ERK1 mRNA could be obviously seen in control and TGF-β1 stimulus group. After the intervention of different concentration of curcumin, the expression of PDGF-BB,PDGFRβ and ERKt mRNA decreased in a dose-dependent manner. Conclusion：Curcumin could inhibit the expression of PDGF-BB,PDGFRβ and ERK1 which might be the mechanism of action curcumin on anti-fibrosis.

作者赵珍东黄兆胜

机构地区广东食品药品职业学院广州中医药大学

出处《中药材》 CAS CSCD 北大核心 2009年第5期732-735,共4页 Journal of Chinese Medicinal Materials

关键词姜黄素肝星状细胞血小板衍生生长因子-BB 细胞外信号调节激酶 Curcumin Hepatic stellate cells Platelet-derived growth factor-BB Extracellular signal-regulated kinase

分类号 R286.02 [医药卫生—中药学]

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