应用基因芯片技术初步探讨散发性甲状旁腺腺瘤基因表达谱

Preliminary study on gene expression profiles of sporadic parathyroid adenoma with application of genechip technology

目的应用基因芯片技术研究散发性甲状旁腺腺瘤基因表达谱的改变，为进一步阐明其早期诊断和发病机理奠定基础。方法抽提正常甲状旁腺和甲状旁腺腺瘤组织中的mRNA来制备探针，经杂交、洗涤后，通过计算机扫描分析正常甲状旁腺和甲状旁腺腺瘤组织基因表达谱的差异情况。结果腺瘤组与正常组的ratio值相比，如以2倍为界，有2302条基因表达发生明显变化，其中1012条基因表达水平明显上调，1290条明显下调。以5倍为界，有10条基因上调，27条下调。基因的功能涉及运输、细胞黏附、信号转导、转录和离子结合等。结论高通量基因芯片技术筛选出大量的散发性甲状旁腺腺瘤相关基因，对这些基因功能的研究可能有助阐明其早期诊断及发病机理。

Objective To investigate the differentially expressed genes in sporadic parathyroid adenomas using genechip and provide basis for further studying the mechanism and diagnosis of sporadic parathyroid adeno- mas. Methods Total RNA was extracted from normal parathyroid tissues and adenomas tissues. The mRNA was used to prepare probes. The mixed probes were hybridized to the eDNA microarray. After washing, the DNA chips were scanned by laser scanner. The acquired image was analyzed. Results Among the 47 000 target genes, 2302 genes were identified in sporadic parathyroid adenomas tissues that had at least two-fold changes compared to normal parathyroid tissues, and 1012 genes up-regulated and 1290 down-regulated were found in this study. Of I0 genes up-regulated and 27 down-regulated, the changes were over five-fold. The function of these changed genes was involved in transport, cell adhesion, signal transduction, transcription, ion binding and so on. Conclusions Many parathyroid adenomas associated genes have been screened by high-throughout gene chip method, Which may help to identify the mechanism and diagnosis of sporadic parathyroid adenomas.