通关藤提取物体外对Jurkat、Raji、RPMI8226细胞的抑制作用研究

Effect of extract from Marsdenia Tenacissima on Jurkat,Raji and RPMI8226 cells in vitro

目的探讨通关藤提取物对Jurkat、Raji、RPMI8226细胞的抑制作用及可能机制。方法以不同浓度通关藤提取物处理Jurkat、Raji、RPMI8226细胞,MTT法检测其对3种细胞的抑制作用,Annexin V/PI双染法观察细胞形态并检测细胞的凋亡率,JC-1染色法检测细胞线粒体跨膜电位(ΔΨm)水平。结果通关藤提取物对Jurkat细胞的抑制作用最强,对RPMI8226细胞最不敏感。25,50μL/mL的通关藤提取物能降低Jurkat、Raji细胞内ΔΨm,并促进其凋亡。100μL/mL的通关藤提取物也可降低RPMI8226细胞ΔΨm而诱导其凋亡。结论通关藤提取物体外对部分淋巴细胞白血病细胞株和骨髓瘤细胞株有不同程度的诱导凋亡作用,可能是通过降低ΔΨm途径触发这些细胞凋亡。

Purpose To investigate the effect and its mechanism of extract from Marsdenia tenacissima on Jurkat, Raji and RPMI8226 cells. Methods MTT method was adopted to observe the inhibiting effect of extract from Marsdenia tenacissima in different concentrations on Jurkat, Raji and RPMI8226 ceils. Cell morphology and apoptosis were measured by Annexin V/PI staining and mitochondrial membrane potential （ΔΨm） was measured by JC-1 assay. Results Extract from Marsdenia tenacissima had significant inhibitive effect on Jurkat ceils, while no obvious inhibition on RPMI8226 cells. Extract from Marsdenia tenacissima with 25 and 50 μL/ mL concentration could decrease cell ΔΨm and induce apoptosis in Jurkat and Raji cells, while extract from Marsdenia tenacissima with 100 μL/mL concentration could also decrease cell ΔΨm and induce apoptosis in RPMI8226 ceils. Conclusion Extract from Marsdenia tenacissima induces apoptosis on some lymphocytic leukemic cell strains and myeloma cell strain in vitro. The apoptosis may be related to downregulating the mitochondrial membrane potential.