Caspase-3在H_2O_2诱导神经细胞凋亡中的作用

Effects of Expression of Caspase-3 on Neuronal Apoptosis Induced by H_2O_2

目的研究caspase-3在H2O2诱发神经细胞凋亡中的作用,以探讨氧化应激损伤诱导神经细胞凋亡的机制。方法用海马神经细胞原代培养技术,采用1mmol/L H2O2诱导建立细胞氧化损伤模型,并观察细胞形态学变化,采用TUNEL法检测H2O2诱导大鼠海马神经元凋亡率;,采用RT-PCR法检测caspase-3 mRNA的表达。结果形态学观察结果显示模型组比海马组细胞损伤程度较严重;与对照组比较,模型组细胞凋亡率及caspase-3 mRNA的表达显著增高(P<0.01)。结论本结果提示,caspase-3可能参与了氧化应激损伤诱导的神经细胞凋亡过程。

Objectives To research the expression of caspase-3 during neuronal apoptosis induced by H2O2 in order to reveal the mechanisms of apoptosis induced by oxidative stress injury. Methods The model of neuronal apoptosis induced by H2O2 was established in the cultured hippocampal neurons. The change in cell morphology was observed. The rate of apoptosis in the hippocampal neurons was detected by TUNEL method. The expression of caspase-3 mRNA was determined by RT-PCR. Results The morphological observation showed that damage of hippocampal neurons was more severe in the experimental group than that in the normal group. The rate of neuronal apoptosis and the expression level of caspase-3 mRNA were significantly higher in the experimental group than that in the normal group （P〈0.01）. Conclusion It is suggested that caspase-3 is involved in the neuronal apoptosis after oxidative stress injury.