摘要
目的:建立龙血竭胶囊的含量测定方法。方法:运用高效液相色谱法(HPLC)对龙血竭胶囊中龙血素B含量进行测定。色谱柱:Kromasil C_(18)柱(4.6mm×200mm,5μ),填充剂为十八烷基硅烷键合硅胶。流动相:乙腈-冰醋酸(1→90)溶液(40:60);流速:1.0ml/mim;检测波长:270nm;柱温:室温。结果:龙血素B的量在0.18～0.90 μg之间呈良好线性关系,平均加样回收率101.5%,RSD=0.76%。结论:所建立的方法可靠,可用于龙血竭胶囊的质量控制。
Objective： To establish the quality standard for Dragon＇s blood and Resina Draconis Capsules. Methods： Determination of Dracorhodin and Loureirin B by HPLC. The HPLC system column （4.6mm×200mm,5μ） , Stuffing： ODS. Mobile phase： the mixture of methyl cyanide： glacial acetic acid（1 →90）=40：60 with a flow rate of 1.0 mL/min and the detection wave of 360nm. Column temperature： room temperature, Results： A good linear relationship between peak area and concentration of Loureirin B was found at the range of 0.18-0.90μg, the average recovery of Loureirin B was 101.5% and RSD was 0.76 %（ n = 5）. Conclusions：The method is reliable, accurate and specific. It can be used for the quality control of Dragon＇s blood and Resina Draeonis Capsules.
出处
《中国医药导刊》
2009年第6期1045-1046,共2页
Chinese Journal of Medicinal Guide
基金
黑龙江省教育厅面上项目(编号:11531346)
