兔蛛网膜下腔出血后脑脊液和血清S100B的变化及其意义

Changes of the S100B protein concentration in serum and CSF after subarachnoid hemorrhage in rabbit model and their significance

目的探讨兔蛛网膜下腔出血(SAH)后血清和脑脊液(CSF)中S100B蛋白的变化及其意义。方法采用枕大池二次注血法制作SAH模型,动物随机分为正常组、穿刺组、盐水对照组和SAH组,正常组于饲养观察3d后取其血清及CSF,其余各组分别于建模后1 h、3 d、5 d、7 d、10 d取血清及脑脊液。应用双抗体夹心ELISA法检测各组血清及CSF中S100B蛋白的浓度。数据结果应用统计软件SPSS13.0进行处理。结果SAH组血清及CSF中S100B蛋白浓度在各个时间点均明显高于其余3组(P=0),并呈现血清S100B蛋白浓度于SAH后1 h即开始升高,3～5 d达到高峰后逐渐恢复,而CSF中S100B则于SAH后1 h升高后稍下降;再于5～7 d第二次达到高峰的变化。盐水组则呈现血清和CSF中S100B蛋白于造模后1 h高于穿刺组及正常组(P<0.05),然后迅速下降至正常。结论SAH后血清与CSF中S100B蛋白浓度呈明显的动态变化。测定血清与CSF中S100B蛋白浓度对判断SAH后血脑屏障(BBB)的病理生理改变具有重要意义。

Objective To discuss the changes of the S100B protein concentration in serum and cerebral spinal fluid （CSF） after subarachnoid hemorrhage （SAH） in rabbit model and their significance. Methods Rabbit SAH model was induced by the cisterna magna puncture and injection two times of autogeneic blood into the cisterna magna. The animals were divided randomly into SAH group, saline group, puncture group and blank group. The serum and CSF were taken in blank group after 3 days＇ breeding. At 1 h, 3 d, 5 d, 7 d and 10 d after the first infusion, the serum and CSF of the other groups were taken. ELISA method was used to detect S100B protein concentration in serum and CSF. The result data was analyzed by software SPSS13.0. Results S100B protein concentration in serum and CSF of SAH group was much higher than that in the other three groups （P=0）. S100B protein concentration in serum ascended from 1 h after SAH, reached the peak at 3-5 d after SAH, and then descended slowly. S100B protein concentration in CSF ascended from 1 h after SAH, then slightly descended, ascended and reached the peak at 5-7 d after SAH, and then descended slowly. S100B protein concentration in serum and CSF of saline group was higher than that in puncture group and blank group from 1 h after model establishment （P〈0.05）. S100B protein concentration of saline group recovered quickly. Conclusion S100B protein concentration in serum and CSF after SAH has obvious changes. S100B protein concentration in serum and CSF after SAH is of great significance to judging pathophysiology of blood-brain barrier.