淫羊藿提取物的雌激素样作用研究

Study on the estrogen-like effects of Epimedium extractive

目的评价淫羊藿提取物的雌激素样作用。方法采用小鼠子宫增重实验、雌激素受体阳性细胞系(MCF-7)细胞增殖及拮抗实验,评价淫羊藿及淫羊藿苷的雌激素样作用及其可能机制。结果与阴性对照组比较,淫羊藿及淫羊藿苷能使子宫系数由0.097±0.008明显增加至0.156±0.018及0.152±0.016,且用药前、后动物体重由(18.9±1.3)g分别增至(19.8±1.3)g及(20.03±1.3)g,增幅明显高于阴性对照组(P<0.05);淫羊藿苷可使血清雌激素E2由(132.22±11.45)ng/L增高至(147.73±12.43)ng/L,FSH水平由(50.15±3.36)μg/L降低至(44.47±2.46)μg/L(P<0.05),但淫羊藿组雌激素E2水平无明显改变;在24、48、72 h,淫羊藿及淫羊藿苷对MCF-7细胞的增殖均有明显的促进作用(P<0.05),分别由0.238±0.043、0.387±0.051、0.563±0.083增至0.362±0.045、0.521±0.046、0.648±0.043及0.324±0.043、0.465±0.034、0.622±0.079;72 h加入ICI后,细胞增殖明显受到抑制,细胞增值率分别降至99.461%、94.434%。结论淫羊藿及淫羊藿苷具有雌激素样作用,且其促细胞增殖作用可能是通过雌激素受体(ER)介导产生的。

Objective To investigate the estrogen-like effects of Epimedium extractive. Methods By using the uterus growth test in mice and test of MCF-7 proliferation and adding estrogen receptor antagonist Icariin, we evaluated the estrogenic effects of Epimedium and Icariin and the possible mechanism. Ftesults Epimedium and lcariin increased significantly the uterus coefficient in mice from 0.097 ± 0. 008 to 0.156 ± 0.018 and 0.152 ± 0.016, respectively; the weight increased significantly from （18.9±1.3）g to （19.8±1.3）g and （20.03± 1.3）g （P〈0.05）. Icariin incrcased significantly the serum level of E2 from （132.22 ± 11.45）ng/L to （147. 73 ± 12.43）ng/L and decreased FSH level from （ 50. 15 ± 3.36 ） μg/L to （44.47 ± 2.46） μg/L （ P 〈 0.05）, but there was no significant difference in Epimedium group. Thcrc was obviously enhanced proliferation in MCF-7 in 24, 48 and 72 h （P〈 0.05）, from 0.238±0.043, 0.387±0.051 and 0.563±0.083 to 0.362±0.045, 0.521±0.046 and 0.648±0.043 as well as 0. 324±0. 043, 0.465±0.034 and 0.622±0.079, respectively. However, the cell proliferation was inhibited significantly by Icariin added in 72 h, the proliferation ratio decreased to 99. 461% and 94. 434%, respectively. Conclusion Epimedium and Icariin have estrogen-like effects and all of their effects arc realizcd through the estrogen receptor.