离子注入对花生的生物学效应

Biological Effect of Energy Ion Implantation on Peanut (Arachis hypogaea L.)

取山东烟台选育的鲁花10号花生(Arachis hypogaea L.)种子,用离子注入机分别注入0(即对照组)、1×1012Cu、1×1011Cu、4×1011P、9×1011P。结果发现,注入9×1011P的花生,其植株的叶片数目、开花数目等均优于其他组;注入1×1012Cu的花生,单株结实数、百粒湿重、单株产量等最优;聚丙烯酰胺凝胶电泳对幼苗叶片进行的酯酶同工酶分析表明,注入1×1011Cu、9×1011P、4×1011P的花生幼苗的酯酶活性增大。综合分析的结果显示,以9×1011P和1×1012Cu组所诱导产生的优良生物学性状较多,可以进一步对其进行选育。

The effects of energy ion implantation with different ions at different concentration on the biological mutagenesis were observed in details and proved by the analysis of data derived from different groups of peanut （Arachis hypogaea L. ）. At dose of 9×10^11 P ion beam implantation, the peanut plant grew with the most leaves and flowers per plant among the experiment. At dose of 1×10^12 Cu implantation, the plant grew with the most fruits per plants and the highest 100-seed weight in the experiment. Polyacrylamide gel electrophoresis of esterase isozymes on leaves of the seedlings indicates that the enzyme activity were all increased in plants with ion implantation at dose of 1 ×10^11 Cu, 9×10^11Pand 4×10^11 P. It was found that the 9×10^11 p and 1×10^12 Cu treatments showed better characters in treated generation one.