基于BP神经网络的半夏泻心汤及其类方性味药组对小鼠小肠运动的影响

Investigation of the effects on small intestine motility of different ingredient groups in Banxiaxiexin recipe and Banxiaxiexin-allied recipes by using the BP neural network technique

目的:探讨半夏泻心汤及其类方中性味药组对小鼠小肠运动的影响。方法:采用均匀设计法对半夏泻心汤及其类方进行拆方,测定各配比组合对正常对照、小肠运动抑制模型、小肠运动亢进模型小鼠小肠推进率的影响;基于BP神经网络,针对实验数据建立药味与药效指标的非线性映射模型;在模型基础上,固定各药组中药物的用量比例,进而分别筛选各药组在全方背景下的量效关系。结果:在半夏泻心汤及其2个类方中,半夏和干姜对小鼠肠运动抑制及亢进模型具有双向调节作用;干姜对正常小鼠的肠运动有明显的促进作用,半夏的作用不明显。结论:在肠运动模型,半夏的药效学作用与半夏泻心汤具有高度的一致性,提示半夏可能为半夏泻心汤之君药;但仅从小肠推进率的药效作用,尚难归纳出生姜和甘草的配伍角色。

Objective： To investigate the effects of the ingredient groups with different pharmacological features in Banxiaxiexin recipe and Banxiaxiexin-allied recipes on small intestine motility. Method： The homogeneous design method was adopted to classify the ingredients of Banxiaxiexin recipe and Banxiaxiexin-allied recipes. The effects of different ingredient matching and combinations on the impelling ratio of the small intestine were observed in the model mice in the normal control group, intestinal motility inhibition group and the intestinal motility hyperactivity group. The BP neural network technique was used to establish the nonlinear mapping model of the recipe ingredients and the criteria for their efficacy on the basis of the experimental data. Based on the nonlinear mapping model, the ratios of different ingredients in different ingredient groups were set up and the dose-effect relationships between different ingredient groups and the entire recipes were investigated. Result： In the Banxiaxiexin recipe and the two Banxiaxiexin-allied recipes, Rhizoma Pinelliae and Rhizoma Zingiberis in combination showed bidirectional regulative effect on intestinal motility in the mice in the intestinal motility inhibition group and the intestinal motility hyperactivity group, and Rhizoma Zingiberis alone had marked promotive effect on mouse intestinal motility while Rhizoma Pinelliae alone had no obvious such effect. Conclusion： In the experiments with the mice in different intestinal motility model groups, Rhizoma Pinelliae showed a pharmacodynamic action highly accordant with the one it showed in the entire Banxiaxiexin recipe, which suggests that Rhizoma Pinelliae might be the principal ingredient of the Banxiaxiexin recipe. As far as Rhizoma Zingiberis and Radix Glycyrrhizae are concerned, it was difficult to generalize their roles in the relevant recipes according to the results from the experiments on small intestine motility.