牛磺酸对局灶性脑缺血大鼠神经干细胞增殖的影响

Effect of Taurine on proliferation of neural stem cell after cerebral ischemia in rats

目的:研究牛磺酸对成年大鼠脑缺血后缺血侧神经干细胞增殖的影响及治疗缺血性脑损伤的作用机制。方法:采用开颅热凝闭法制作大脑中动脉阻塞(MCAO)模型,随机分为正常组、假手术组、模型组与牛磺酸组,采用腹腔注射每天给予牛磺酸,采用5-溴脱氧尿苷(Brdu)免疫组化法观察脑缺血后3,7,14d与21d4个不同时相Brdu阳性细胞表达的数量。结果:脑缺血后不同时相缺血侧皮质、海马齿状回和纹状体均有Brdu阳性细胞表达,其中7d时各区的阳性细胞数量明显增加,分别为(18.65±4.38)、(25.63±3.58)、(19.46±2.98),具有统计学意义(P<0.05);不同时相牛磺酸组Brdu阳性细胞表达与模型组比较明显增加(P<0.05)。结论:牛磺酸可促进缺血后脑区Brdu阳性细胞数量的增加,提示牛磺酸对治疗脑缺血有重要作用。

Objective： To study the influence of Taurine on proliferation of neural stem cells after focal cerebral ischemia in rats and to explore the mechanism of taurine in treating ischemic brain injury. Methods： The model of middle cerebral artery occlusion was made by using opening ranium hot coagulation in 80 rats, who were divided randomly into normal group, sham operate group, model group and taurine group. Taurine was administrated once dally by i. p. in taurine group, and immunohistochemistry method was used to observe the number of Brdu positive cells on the 3rd, 7th, 14th and 21st day after ischemia. Results： There were Brdu positive cells in the cortex, hippocampal dentate gyrus （DG） and striatum at different time points after cerebral ischemia, and the number of Brdu positive cells in various brain regions increased significantly on the 7th day as （18.65 ±4.38）, （25.63±3.58）, （19.46 ±2.98） and having statistical significance （P〈0.05）. It was also found that the number of Brdu positive cells in different phase taurine group was significantly more than that in the model group （P〈0.05）. Conclusion： Taurine can increase the number of Brdu positive cells in some brain regions after focal cerebral ischemia, which has the important effect on treating ischemic brain injury.