中药抗菌复方Ⅰ的筛选及甲氧苄啶对其抗菌增效的研究

Screening of Chinese Herbal CompoundⅠand study on synergitic antibacteria actions of Trimethoprim combined with it

目的:筛选出由黄芩、秦皮、白头翁和苦参组成的中药抗菌复方Ⅰ,并确定甲氧苄啶对其体外抗菌增效的最优添加剂量。方法:按照正交试验L9(34)设计9个组方,采用二倍稀释法测定各组方对金黄色葡萄球菌、大肠杆菌和沙门氏菌的体外抗菌效果;采用平板计数的方法,测定中药抗菌复方Ⅰ与甲氧苄啶联用作用于3种细菌1、2、4和8h后的细菌数,并计算杀菌率,并用最小二乘法对杀菌率进行拟合,计算甲氧苄啶的最优添加剂量。结果:筛选出黄芩、秦皮、白头翁和苦参按照1:4:1:2的比例组成中药抗菌复方Ⅰ,甲氧苄啶对其体外抗菌增效的最优添加剂量是2mg/g。结论:甲氧苄啶对由黄芩、秦皮、白头翁和苦参4味中药组成中药抗菌复方Ⅰ的抗菌增效作用显著。

Objective： Chinese Herbal Compound I were determined by screening of Radix Scutellariae, Cortex Fraxini, Radix Pulsatillae and Radix Sophorae Flavescentis. The optimal dose of Trimethoprim which combined with it against bacteria in vitro were determined. Method： The composing prescriptions were designed by L9（3^4） orthogonal test.The antibacterial effect of Staphylococcus aureus, Escherichia Coli and Salmonella in vitro were evaluated by double dilution method. The number of Staphylococcus aureus, Escherichia Coli and Salmonella which had been inhabited by Chinese Herbal Compound Ⅰ combined with Trimethoprim for 1, 2, 4h and 8h, were enumerated by Plate Counts Methods. Then the killing rate of Chinese Herbal Compound I combined with Trimethoprim were calculated. The experimental data were fitted by least squares method and the optimal dose of Trimethoprim were determined. Result： The Composition of Chinese Herbal Compound Ⅰ were mixed in the proportion of 1 to 4 to 1 to 2 with Radix Scutellariae, Cortex Fraxini, Radix Pulsatillae and Radix Sophorae Flavescentis. The optimal dose of Trimethoprim was 2mg/g. Conclusion： Antibacterial action and synergistic action of Chinese Herbal Compound Ⅰ were significant.