rhG-CSF促进不同性别大鼠缺血后肢血管新生的作用

THE PROMOTION OF NEOVASCULARIZATION OF rhG-CSF IN DIFFERENT-GENDER RATS WITH ISCHEMIC HIND LIMP

目的观察重组人粒细胞集落刺激因子（rhG-CSF）促进不同性别成年大鼠缺血后肢血管新生作用。方法健康成年雄性和雌性Wistar大鼠各20只,均切断左侧股动脉及其分支,制作大鼠后肢缺血模型。成模后雄性大鼠和雌性大鼠各随机分为2组,即对照组和实验组,每组10只。实验组皮下注射rhG-CSF,对照组皮下注射等量生理盐水。于术前和注射rhG-CSF及生理盐水后第5天检查血白细胞数;术后4周取内收肌行病理切片检查,用图像分析系统统计血管密度,用免疫组化法标记内收肌中血管内皮细胞生长因子（VEGF）阳性表达的血管,用图像分析系统统计血管数。结果实验组大鼠血白细胞数、血管密度及VEGF阳性表达的血管数均显著高于对照组（t=4.318-4.981,P〈0.05）;雄性实验组和雌性实验组之间,雄性对照组和雌性对照组之间差别均无统计学意义（P〉0.05）。结论皮下注射rhG-CSF可促进缺血肢体血管新生,改善肢体缺血状态,而对血管新生作用与性别无关。

Objective To observe recombinant human granulocyte colony stimulating factor （rhG-CSF） in promotion of angiogenesis in different-gender adult rats with ischemia of hind limb. Methods Healthy adult male and female Wistar rats, 20 for each gender, were enrolled. A hind-limp ischemic model was established by cutting off the left femoral arteries and their bran- ches. The rats were equally randomized to two groups： control group and experimental group. Subcutaneous injection of rhG-CSF was offered to the experimental, and equal volume NS to the control. WBC was detected before and five days after the injection; adductor was collected four weeks later and sent for pathology. The density and number of blood vessels were detected by image analysis system; the VEGF of the adductor by immunohistochemistry. Results The white blood count, density and positive VEGF were significantly higher in the experimental than that in the control, the differences between the two groups were significant （t=4. 318-4. 981, P〈0.05） ; but no significant difference was noted in terms of the gender （P〉0.05）. Conclusion Sub cutaneous injection of rhG-CSF can facilitate angiogenesis of ischemic limb, improve ischemic status of the limb, the effects do not show association with the gender.