摘要
目的研究β1整合素反义寡核苷酸(ASODN)对人结肠癌HT-29细胞侵袭的抑制作用。方法以脂质体为载体将硫代磷酸修饰的β1整合素ASODN及无义寡核苷酸(NSODN)转染人结肠癌HT-29细胞,通过RT-PCR及Western-Blot试验,分别测定ASODN组、NSODN组和空白对照组β1整合素mRNA和蛋白的表达,MTT法和Transwell小室法分别测定其黏附力和侵袭力。结果与NSODN组和空白对照组相比较,ASODN组中β1整合素mRNA和蛋白表达强度明显降低;ASODN组和NSODN组转染的人结肠癌HT-29细胞侵袭力均下降,但前者下降较为明显。结论β1整合素ASODN转染HT-29细胞后,通过降低其β1整合素mRNA和蛋白表达水平,从而抑制其对细胞外基质的侵袭。
Objective To study the inhibitory action of integrin-β1 antisense oligonucleotide on invasion of human colon cancer cells. Methods Methods Using liposome as a vecter, integrin β1 antisense oligonucleotide and non-oligonucleotide were transfected into human colon cancer cells, which were then randomized to ASODN group, non-antisense oligodeoxynucleotide group (NSODN) and control group. The expressions of integrin-β1 mRNA and protein were determined by RT-PCR and Western Blot, and the adhesion and invasion were measured by MTT and Transwell ventricle. Results Compared with the NSODN and blank control, the expressions of integrin-β1 mRNA and protein in the ASODN were obviously lower; the invasiveness of human colon cancer cells transfected by integrin-β1 antisense oligonucleotide and non-oligodeoxynucleotide was decreased, but the former was more obviously. Conclusion Integrin β1 antisense oligonucleotide can inhibit invasiveness of extracellular matrix by decreasing the expression level of integrin-β1 mRNA and protein after transfecting human colon cancer cells.
出处
《齐鲁医学杂志》
2009年第4期286-288,共3页
Medical Journal of Qilu