MMP-2和TIMP-2对结核性和恶性胸腔积液鉴别诊断价值

THE VALUE OF MATRIX METALLOPROTEINASE 2 AND TISSUE INHIBITOR OF METALLOPROTEINASE 2 IN DIFFERENTIAL DIAGNOSIS BETWEEN TUBERCULOUS AND MALIGNANT PLEURAL EFFUSION

目的探讨胸腔积液中基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶组织抑制因子-2(TIMP-2)水平对结核性胸腔积液及恶性胸腔积液的鉴别诊断价值。方法采用ELISA法,检测34例结核性胸腔积液、35例肺部肿瘤并胸腔积液病人胸腔积液中MMP-2和TIMP-2水平,并进行比较。结果恶性胸腔积液组MMP-2和TIMP-2水平均明显高于结核性胸腔积液组,差异有显著性(t=7.099、8.515,P<0.01)。两组MMP-2/TIMP-2比值差异无显著性(P>0.05)。诊断特异度与灵敏度TIMP-2均大于MMP-2。结论MMP-2和TIMP-2可以作为结核性胸腔积液和恶性胸腔积液的鉴别诊断标志。

Objective To assess the value of of matrix metalloproteinase 2 （MMP 2） and tissue inhibitor of metalloproteinase 2 （TIMP-2） in differentiating tuberculous from malignant pleural effusion. Methods Using ELISA, the levels of MMP- 2 and TIMP 2 in pleural effusion in 34 patients with tuberculous pleural effusion and 35 with malignant pleura1 effusion were detec ted and compared. Results The MMP-2 and TIMP-2 levels in malignant pleural fluid were markedly higher than that in tuberculous plenral fluid, with a significant difference between them （t=7. 099,8.515; P〈0.01）, while the difference of MMP-2/TIMP- 2 ratio was not significant （P〉0.05）. The sensitivity and specificity of TIMP 2 were higher than that of MMP-2. Conclusion MMP-2 and TIMP -2 can be used as differential diagnostic markers in tuberculous pleural effusion and malignant pleural effusion.