人参总皂苷对K562细胞STAT5表达的影响

Effect of total saponins of panax ginseng on expression of STAT5 in K562 cells

目的探讨人参总皂苷(TSPG)对人红白血病细胞株(K562细胞)信号转导与转录因子5(STAT5)表达的影响。方法紫外-可见分光光度法测定血红蛋白含量及Wright′s染色法检测TSPG诱导K562细胞向红系细胞分化的变化;免疫细胞化学观察TSPG作用前后K562细胞STAT5表达的变化;以ELISA和Western blotting法检测TSPG处理前后K562细胞的细胞质和细胞核中STAT5含量的变化。结果TSPG(200mg/L)诱导K562细胞6h、12h、24h后,随着TSPG作用K562细胞时间的延长,细胞中血红蛋白的含量逐渐增加;而加入AG490(40μmol/L)部分抑制了K562细胞中血红蛋白含量的增加。免疫细胞化学染色可见对照组K562细胞的细胞质和细胞核着色浅淡;经TSPG 200mg/L作用12h后,K562细胞的细胞质和细胞核着色明显加深,表达增强,与对照组相比差异有统计学意义(P<0.05);TSPG(200mg/L)诱导K562细胞6h、12h、24h后,随着TSPG作用K562细胞时间的延长,细胞质和细胞核中STAT5的表达逐渐增强。结论TSPG能增强K562细胞STAT5的表达,这可能是TSPG诱导K562细胞向红系细胞分化的作用机制之一。

Objective To investigate the effect of total saponins of panax ginseng on expression of STAT5 in K562 cells. Methods Spectrophotometry and Wright＇s staining were used to determine if TSPG induce K562 cells to differentiate into erythroid lineage cells. The expression of STAT5 protein in K562 cells was investigated by immunocytochemistry; STATS protein in cytosol fraction and cellular nucleus protein extracts of K562 cells were determinated by Elisa and Western blotting analysis. Results The level of hemoglobin in K562 cell induced by 200μg/ml TSPG is increased in time-dependent manner, AG490（40μmol/L） partly inhibited the rise of hemoglobin level in K562 cells. The cytoplasma and nucleus of K562 cell in control group is weakly stained for STAT5 ; alter stimulated by 200μg/ml TSPG for 12h, both eytoplasma and nucleus of K562 cell is darkly stained（P 〈0. 05 ）. Compared with control group, the amount of STAT5 in cytoplasma and nucleus of K562 cell induced by 200μg/ml TSPG is increased in time-dependent manner. Conclusion TSPG can enhance the expression of STATS in K562 cell, which may be one of mechanisms by which TSPG induces K562 cells to differentiate into erythroid lineage cells.