VEGF基因多态性与子痫前期易感性的研究

Analysis on gene polymorphism of vascular endothelial growth factor and susceptibility of preeclampsia patients

目的:探讨血管内皮细胞生长因子(VEGF)基因多态性与子痫前期遗传易感性的关系。方法:对128例子痫前期患者和231例对照者进行VEGF基因+936C/T多态性分析;Logistic回归模型分析基因多态性与子痫前期遗传易感性的相关性;ELISA法检测血浆VGEF浓度。结果:VEGF+936等位基因C、T和各基因型在子痫前期组和对照组分布的差异有统计学意义(P<0.05),其中等位基因T使子痫前期患病风险为C等位基因的2.310倍;TT基因型、CT基因型者子痫前期患病风险为CC基因型的2.412倍。此外,+936TT、CT基因型者VEGF水平低于CC基因型(P<0.01),TT、CT基因型中子痫前期患者VEGF水平低于正常对照组(P<0.05)。结论:VEGF基因+936基因多态性与子痫前期的发生存在关联,T等位基因和CT/TT基因型携带子痫前期的患病风险增加,可能与VEGF的表达水平下降相关。

Objective： To explore the relationship between gene polymorphism of vascular endothelial growth factor （VEGF） and susceptibility of preeclampsia patients. Methods： Gene polymorphism analysis of VEGF ＋ 936 C/T was conducted in 128 cases with preeclampsia and 231 healthy cases, the correlation between gene polymorphism and susceptibility of preeclampsia was analysed by logistic regression analysis ; the level of VEGF was detected by enzyme - linked immunosorbent assay. Results ： There was significant difference in the distribution frequency of allele C, T on VEGF ＋ 936 gene and genotypes between preeclampsia group and control group （ P 〈 0. 05 ）, the risk ratio of preeclampsia cases with allele T was 2.310 times higher than that with allele C; the risk ratio of preeclampsia cases with CT/TT genotypes were 2. 412 times higher than that with CC genotype. Additionally, the level of VEGF in cases with ＋ 936T allele and CT genotype was lower than that with CC genotype （P 〈0. 01 ） ; for the cases with CT/TT genotypes, the level of VEGF in preeclampsia group was lower than that in control group （P 〈 0. 05 ） . Conclusion ： Gene polymorphism of VEGF ＋ 936 C/T is related to preeclampsia, the cases with allele T and CT/TT genotypes have high risk of preeclampsia, which maybe related to down regulation of VEGF.