花生壳提取物的体外抗氧化活性研究

Study on Antioxidant Activity of Peanut Hull Extracts in Vitro

为研究花生壳提取物的体外抗氧化活性,测定了花生壳提取物的还原能力、抗脂质过氧化作用、抑制油脂过氧化作用以及清除超氧阴离子自由基、羟基自由基、DPPH自由基的能力,并与抗坏血酸进行了比较。结果表明,花生壳提取物具有较好的总抗氧化活性和总还原性,能抑制脂质过氧化和油脂过氧化作用以及清除自由基。它对DPPH自由基、羟基自由基、超氧阴离子自由基的IC50分别为27.82μg/ml1、83μg/ml8、9μg/ml。研究表明,花生壳提取物具有较强的综合抗氧化能力,是一种很有开发价值的天然抗氧化剂。

To study the antioxidant activity of extraction of peanut hull extracts （PHE） in vitro,the antioxidant activity of the PHE was analyzed by using different assays, such as reducing power, anti - lipid peroxidation, inhabit oils peroxidation, superoxide radical scavenging assay, hydroxyl radical scavenging assay, DPPH radical scavenging assay. The results showed that the PHE was proved with total antioxidant activity in evidence, effective reducing power, inhibition of lipid eoxidation and free radical, scavenging activity. The IC50 values of inhibition for DPPH radical, hydroxyl radical, superoxide anion radical were 27.82μg/ml, 183μg/ml, 89μg/ml, respectively. The PIlE possesses was rather high and multiply antioxidant activity and was a promising natural antioxidant.