摘要
以马铃薯品种早大白、黄麻子、克新13、荷兰15、大西洋为试验材料,提取基因组DNA,进行SSR标记,并将标记产物进行聚丙烯酰胺凝胶电泳,比较不同电泳参数(聚丙烯酰胺凝胶的浓度、电泳电压、染色方法)对电泳结果的影响。确定了适合马铃薯SSR标记的电泳检测体系,即聚丙烯酰胺凝胶的浓度为12%,电泳电压为170V,染色方法为溴化乙锭染色。
The genomic DNA was extracted from five potato varieties, Zaodabai, Huangmazi, Kexin 13, Favorita and Atlantic. The effects were compared of the electrophoresis parameters of gel concentration, voltage and stain substrate on the outcomes of electrophoresis by polyacrylamide gel. The optimal eleetrophoresis conditions for potato SSR markers were 12% gel concentration, 170 V voltage and EB as staining reagent.
出处
《中国马铃薯》
2009年第3期143-147,共5页
Chinese Potato Journal
基金
黑龙江省科技厅国际合作项目资助(WC05B08)
