厌氧蛋白质氨化细菌筛选及对酸化液pH的影响

Effect of acidizing fluid pH on anaerobic protein-annonifying bacteria

将筛选的5株R/r大于3,酶活力200～300U·mL-1,氨态氮产生量大于100mg·L-1,且适宜在起始pH5.0～6.0、35℃下生长的厌氧蛋白质氨化细菌应用于pH5.5的酸化液。其中产低温酸性蛋白酶的k3B37和产低温偏酸性蛋白酶的m1B94菌株,生物量为0.315和0.643时产酶活性达到302.2、271.7U·mL-1;使酸化液氨态氮含量分别提高了373.47、302.89mg·L-1,从而将酸化液pH调至7.29、7.03,为产甲烷菌创造适宜生长、产气条件。

Five anaerobic protein-ammonifying bacterias were isolated. The strains could produce larger hydrolysis cycles with R/r more than 3, their high enzymes activity reached 200-300 U·mL^-1, ammonia-N production were higher than 100 mg·L^-1, and grew well in initial pH 5.0-6.0, temperature 35 ℃. The islations were applied at pH 5.5 acidizing fluid. The results showed that biomass of k3B37 produced low temperature acid protease and m1B94 produced low temperature partial acid protease were respectively 0.315, 0.643. Their enzyme activities reached 302.2 and 271.7 U·mL^-1. The strains made the contents of nitrogen ammunia increased by 373.47, 302.89 mg·L^-1 of acidizing fluid. Consequently, pH of acidizing fluid were adjusted to 7.29 and 7.03, respectively, the condition was suitable for growth and producing gas of methanogen.