克雷伯氏菌N-5对三苯基锡的降解性能

Degradation characteristics of triphenyltin by Klebsiella sp. N-5 strain

通过耐受性实验,得到三苯基锡(TPT)高耐受性细菌N-5、N-6和酵母菌Ja、Jc.经初步测试,发现克雷伯氏菌N-5菌株的降解性能较为理想.性能研究表明,当菌龄为36 h且水样中菌质量浓度、TPT质量浓度、外加碳源葡萄糖质量浓度分别为14 g/L、5、5 mg/L时,该菌对TPT的降解效果最好,5 d降解率达到50%左右.扫描电镜观察显示,该菌在有TPT存在的环境中,细胞发生明显变化,其变化程度随着TPT质量浓度的提高和处理时间的延长而加大,刺激强度较大时细胞变皱.且该菌体有集中起来抵御不良生长环境的自我保护机制.

Two bacterial strains N-5, N-6 together with two yeasts Ja and Jc which possessed the properties of toxicity resistance to triphenyltin were isolated. The preliminary experimental analysis showed that strain N-5 which was identified as KlebsieUa sp. exhibited much better degradation ability of TPT. After treating TPT for five days, this strain could degrade 50% TPT when the microorganism age was 36 h and the concentrations of bacteria, TPT and glucose were 14 g/L, 5 and 5 mg/L, respectively. Scan Electronic Microscope experiments showed that bacterial ceils changed a lot in TPT polluted environment, and greater change would occur as time passed came crumpled because of long time contact with high and at higher concentration of TPT. The cells beconcentration of TPT. At the same time, the ceils would aggregate together to withstand bad environment to protect themselves.