摘要
利用一种单胺化的半乳糖衍生物,在炭化二亚胺的催化下,对葡萄糖氧化酶(GOD)进行糖苷化修饰,并利用蓖麻凝集素亲和层析分离已修饰的和未修饰的酶.修饰后的酶经荧光光谱和吸收光谱分析,证实其结构发生了变化.酶活性检测证明了修饰后GOD的热稳定性增加,在较高温度如在55℃下酶活性提高了7.28倍.本研究为拓宽GOD的应用奠定了基础.
A monoaminated galactose derivative was attached to glucose oxidase （GOD） to modify it via a carbodiimide-catalyzed reaction. Ricinus agglutinin affinity chromatography was first adopted to soparate the modified and non-modified enzyme. The modified GOD was confirmed by absorption and fluorescence spectroscopy. The thermostability of modified GOD was enhanced, based on the examination of enzymatic activity. Under a higher temperature, such as at 55℃, the catalytic activity of modified GOD has been enhanced as high as 7.28 folds.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2009年第3期305-309,共5页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
暨南大学自然科学基金(640073)
关键词
葡萄糖氧化酶
半乳糖
化学修饰
热稳定性
glucose oxidase
galactose
chemical modification
thermostability
