声敏剂血卟啉对COC1/DDP细胞顺铂敏感性的影响及机制探讨

A Discussion on Effect and Mechanism of Haematoporphyrin to Chemotherapy Sensitivity of Cisplatin on COC1/DDP

目的探讨声敏剂血卟啉对COC1/DDP细胞顺铂敏感性的影响及其机制。方法将人卵巢癌顺铂耐药细胞株COC1/DDP分为A、B、C、D4组(分别为对照组、单纯顺铂组、血卟啉+超声组、顺铂+血卟啉+超声组),观察各组处理后癌细胞的增殖、细胞克隆形成情况,联合用药效果及bcl-2和bax表达的变化。结果D组COC1/DDP细胞增殖抑制明显,细胞凋亡率升高,均与其他组差异有统计学意义(P<0.05),bcl-2蛋白阳性表达率在C、D组显著降低(P<0.05),A、B组差异无统计学意义(P>0.05)。bax蛋白阳性表达率在各组表达差异无统计学意义(P>0.05)。结论声动力疗法联合顺铂能有效地增强对耐药卵巢癌细胞的杀伤能力,减少癌细胞的生成。

Objective To determine whether sonodynamic treatment （SDT） can enhance chemotherapy effect of cisplatin on ovarian cancer cells. Methods Drug resistance ovarian cancer cells COC1/DDP were divided into 4 groups randomly： cisplatin （ DDP ） group, haematoporphyrin（ Hp ） ＋ Ultrasound group, cisplatin （ DDP ） ＋ haematoporphyrin （Hp） ＋Ultrasound group and control group. Cell proliferation, apoptosis, protein bcl-2 and bax expression were observed. Results In DDP＋ Hp＋ Ultrasound group, cell proliferation was greatly inhibited, COC1/DDP cell clone efficiency was the lowest（P〈0.05）. Bcl-2 expression in Hp＋ Ultrasound group and DDP＋ Hp＋ Ultrasound group deceased significantly （P〈0. 05）, while there were no obvious difference in control goup and cisplatin group. We did not observe great difference in protein bax expression in any group （P〉0. 05）. Conlusion SDT could effectively enhance cell killing ability of cisplatin on drug resistance ovarian cancaer cells.