EphB2和EphrinB2及EphrinA1在喉癌组织中的表达及意义

The expression and significance of EphB2,EphrinB2 and EphrinA1 in laryngeal carcinoma tissues

目的探讨EphB2、EphrinB2和EphrinA1与喉癌发生发展的关系及探讨喉癌的发病机制。方法应用流式细胞术检测喉癌组织、癌旁组织及喉部正常黏膜组织的EphB2和EphrinA1、EphrinB2蛋白表达的含量。基因蛋白以荧光指数(FI)>1.0为阳性表达,FI≤1.0为阴性表达。结果EphB2蛋白、EphrinB2蛋白、EphrinA1蛋白在喉癌组织中的阳性表达率分别高于癌旁组织和正常喉黏膜组织(P<0.05或<0.01)。在喉癌组织中,EphB2蛋白、EphrinB2蛋白、EphrinA1蛋白在淋巴结转移组的阳性表达率分别高于无淋巴结转移组(P<0.05);临床Ⅲ、Ⅳ期组的阳性表达率分别高于临床Ⅰ、Ⅱ期组(P<0.05或<0.01)。喉癌组织中EphB2蛋白与EphrinA1蛋白、EphrinB2蛋白有多元线性回归关系,且均为正相关(P<0.01)。结论EphB2蛋白和EphrinA1、EphrinB2蛋白高表达可能与喉癌的发生、发展有关。

Objective To investigate the relationship between the expression of EphB2, EphrinB2, EphrinA1 and the pathogenesis of laryngeal carcinoma, and to explore the mechanism of pathogenesis of laryngeal carcinoma. Methods The expression of EphB2, EphrinB2 and EphrinA1 protein with the flow eytometere Fluorescence index was defined as the quantitative expression index of EphB2, EphrinB2 and EphrinA1 protein. The FI value over 1.0 would be considered as positive expression, otherwise,as negative expression. Results The qualitative expressions of EphB2, EphrinB2 and EphrinA1 in laryngeal carcinoma tissues were obviously higher than those in para-carcinoma and in normal laryngeal mucosa tissues respectively （ P 〈 0.05 or 〈 0.01 ）, in laryngeal carcinoma tissues, the qualitative expressions of EphB2, EphrinB2 and EphrinA1 with positive metastasis were remarkably higher than those without metastasis respectively（ P 〈 0.05 or 〈 0.01 ）, which in clinical stage Ⅲ, Ⅳ were significantly higher than those in clinical stage Ⅰ , Ⅱ respectively（ P 〈0.05 or 〈0.01 ）. There was a multiple linear regression correlation in the expression of EphB2, EphrinB2 and EphrinA1 in laryngeal carcinoma tissues, and which was a positive correlation. Conclusion The high expression of EphB2, EphrinB2 and EphrinAl may be related with the pathogenesis and development of laryngeal carcinoma.