腹泻型肠易激综合征大鼠模型的建立

Inducing of irritable bowel syndrome with diarrhea in rats

[目的]在传统束缚应激+番泻叶灌胃方法的基础上进行改良,旨在建立简便易行的腹泻型肠易激综合征大鼠模型。[方法]将大鼠随机分组,空白对照组(N)未做任何处理。单纯番泻叶组(F)灌服最佳浓度番泻叶水煎剂,不行束缚。单纯束缚组(S)灌服高压消毒饮用水,番泻叶联合束缚应激组(F+S)灌服最佳浓度番泻叶水煎剂,以上两组均行肢体束缚。观察各实验组体质量变化、排便情况等。[结果]N和S无稀便排出,而F、F+S两组均能排出不成形的稀软便,以F+S组明显(P<0.05)。与N组相比较,其余3组大鼠平均体质量增长率均有显著性差异(P<0.01),尤以F+S呈明显负增长趋势(P<0.01)。S与F+S可见免疫器官明显萎缩,脏体指数降低,与N、F两组相比较差异具有显著性(P<0.05)。[结论]肢体束缚应激联合低浓度番泻叶灌胃方法,可以成功复制肠易激综合征(IBS-D)大鼠模型。

[Objective] To establish a stable and easy model of irritable bowel syndrome with diarrhea（IBS-D） in rats on the basis of improved traditional method. [Methods] The rats were randomly divided into some groups： the normal （N, without any intervention）, the Folium Cassiae only （F, oral infusion with Folium Cassiae without restriction）, the restriction only （S）, the Folium Cassiae and restriction （F＋S,oral administration folium cassiae plus restriction）. We observed and recorded the general status, the body weight and passage everyday. [Results] All the groups were in a good status. Group F and F＋S were higher in the percentage of the loose stools than that of other groups, and F＋S was better （/~〈0.05）. The other groups were lower in the percentage of the increased body weight than that of group N （P〈0.01） ,and the growth tendency of group F＋S was appeared as negative increasing obviously （P〈0.01）. [Conclusion] It is proved that the improved method can reproduce IBS-D in rats successfully.