期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

调节糖脂代谢紊乱中药多靶点筛选的研究 被引量：1

Multi-target screening of traditional Chinese medicine with regulating glucose and lipid metabolism disorder
下载PDF
导出
摘要 [目的]观察几种中药成分对人肝癌细胞株Hep-G2糖脂代谢相关基因,包括细胞血管生成素相关生长因子(AGF)、葡萄糖-6-磷酸酶(G-6-Pase)、低密度脂蛋白受体(LDLR)和胰岛素受体底物2(IRS-2)mRNA的影响。[方法]培养人肝癌细胞株Hep-G2,以实时定量逆转录聚合酶链反应(Real Time RT-PCR)法检测中药成分对AGF、G-6-Pase、LDLR和IRS-2 mRNA的影响。[结果]小檗碱可以显著提高Hep-G2细胞AGF和LDLR mRNA的表达,姜黄素可以降低G-6-Pase mRNA的表达。[结论]小檗碱和姜黄素可能通过对糖脂代谢相关基因的调节而对糖尿病有一定的治疗作用。 [Objective] To observe the effect of several tradition Chinese medicines on the glucose and lipid metabolism-related genes of human hepatoma cell line Hep-G2, including cell angiopoietin-related growth factor （AGF）, glueose-6-phosphatase （G-6-Pase）, low densi- ty lipoprotein receptor （LDLR） and insulin receptor substrate-2 （IRS-2）. [Methods] The human hepatoma cell line Hep-G2 was cultured and the impaction of the components of Chinese medicine on AGF, G-6-Pase, LDLR and IRS-2 mRNA expression was detected by real time RT-PCR. [Results] Berberine can improve the AGF and LDLR mRNA expression, and curcumin can reduce the G-6-Pase mRNA expression of Hep-G2 ceil line. [Conelusionl The berberine and curcumin have some certain therapeutic effect on diabetes through regu- lating the related genes with glucose and lipid metabolism.
作者 崔广智 李慧颖 张艳军
机构地区 天津中医药大学中药学院
出处 《天津中医药》 CAS 2009年第3期243-245,共3页 Tianjin Journal of Traditional Chinese Medicine
基金 天津市卫生局资助课题(编号:2005063)
关键词 Hep-G_2细胞 糖脂代谢相关基因 REAL TIME RT-PCR hepatoma cell line Hep-G2 glucose and lipid metabolism-related genes Real Time RT- PCR
分类号 R285.5 [医药卫生—中药学]
  • 相关文献

参考文献10

  • 1Yuichi Oike,Masaki Akao,Kunio Yasunaga et al. Angiopoietin-related growth factor antagonizes obesity and insulin resistance [J]. natrue medicine, 2005,20:400-408.
  • 2方显锋,刘德敏.葡萄糖-6-磷酸酶与糖尿病[J].国外医学（内分泌学分册）,2003,23(5):305-307. 被引量：11
  • 3Soutar AK. Intracellar transport of the low-density lipoprotein receptor[J]. Biochem Soc Trans, 1996,34(8):547-552.
  • 4Taniguchi CM, Emanuelli B, Kahn CR, et al. Critical nodes in signalling pathways: Insights into insulin action [J].Nat Rev Mol Cell Biol,2006,7:85-96.
  • 5Veronique Carriere, Maude Le Gall,Florence Gouyon et al. Intestinal Glucose-dependent Expression of Glucose-6-phosphatase [J]. The Journal of Biological Chemistry,2005,280:20094-200101.
  • 6Perfetti R,Barnett P,Mathur R,Egan JM.Novel therapeutic strategies for the treatment of type 2 diabetes[J]. Diabetes Metab Rev, 1998,14 (3):207-25.
  • 7Salter,AM, Brindley,DN, The biochemistry of lipoproteins [J]. J Inherit.Metab Dis. 1988, 11(Suppl):4-17.
  • 8Spady DK.Hepatic clearance of plasma low denity lipoproteins [J]. Semi Liver Dis,1992,12(4):373-82.
  • 9Withers DJ, Burks DJ, Towery H, et al.IRS-2 coordinates IGF-Ⅰ receptor-mediated beta-cell development and peripheral insulin signaling[J].Nat Gene,1999,23:32-40.
  • 10Withers DJ, Gutierrez JS, Towery H, et al. Disruption of IRS-2 cause type 2 diabetes in mice[J].Nature,1998,391:900-904.

二级参考文献20

  • 1Van de Werve G, Lange A, Newgard C, et al. New lessons in the regulation of glucose metabolism taught by the glucose 6-phosphatase system E J~ ~ Eur J Biochem, 2000,267 : 1533-1549.
  • 2Rajas F, Bruni N, Montano S, et al. The glucose-6 phosphatase gene is expressed in human and rat small intestine:regulation of expression in fasted and diabetic rats[J] . Gastroenterology, 1999,117 : 132-139.
  • 3Gefin I , Noel G , Veiga-da-Cunha, et al. Structure of the gene mutated in glycogen storage disease type I b [ J ].Gene, 1999,227 : 189-195.
  • 4Hombuclde L A, Edgerto D S, Ayala J E, et al. Selective tonic inhibition of G-6-Pase catalytic subunit, but not G-6-P transporter, gene expression by insulin in vivo[J]. Am J Physiol Endocrinol Metab, 2001,281 : E'713- E725.
  • 5Argaud D, Kirby T L , Newgard C B , et al. Stimulation of glucose-6-phosphatase gene expression by glucose and fructose-2, 6-bisphosphate[J]. J Biol Chem, 1997,272 : 12854-12861.
  • 6Ayala J E, Streeper R S, Desgrosellier J S, et al. Conservation of an insulin response unit between mouse and human glucose-6-phosphatnse catalytic subunit gene promoters:transcription factor FKHR binds the insulin response sequence[ J]. Diabetes, 1999,48 : 1885-1889.
  • 7Streeper R S, Eaton E M, Ebert D H, et al. Hepatocyte nuclear factor-1 acts as an accessory factor to enhance the inhibitory action of insulin on mouse glucose-6-phosphatase gene transcription[J]. Proc Nail Acad Sci USA, 1998,95:9208-9213.
  • 8Dickens M, Svitek C A , Culbert A A, et al. Central role for phosphatidylinositide 3-kinase in the repression of glucose-6-phosphatase gene transcription by insulin[J]. J Biol Chem, 1998,273 : 20144-20149.
  • 9Metzger S, Goldschmidt N, Barash V, et al. Interleukin-6 secretion in mice is associated with reduced glucose-6-phosphatase[J]. Am J Physiol, 1997,273(2 Pt 1):E262-E267.
  • 10Combs T P, Berg A H, Obici S, et al. Endogenous glucose production is inhibited by the adipose-derived protein Acrp30[ J]. J Clin Invest,2001,108 : 1875-1881.

共引文献10

同被引文献13

引证文献1

二级引证文献2

天津中医药
2009年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部