几个杨树木质部特异启动子的分离与序列分析

Cloning and Sequence Analysis of Xylem Specific Promoters From Poplar

为了获得能在桑天牛（Apriona germari）寄主树种木质部特异表达的启动子用于害虫的防治,参照美洲山杨（Populus tremuloidMichx.）木质部纤维素合酶基因（PtCesA）序列（GenBank登录号：AF072131）设计引物,分别以三倍体毛白杨-93（Populus tomentosaCarr.-＂93＂）、加杨-1（Populus CanadensisMoench.-＂1＂）、意大利214杨（Populuscanadensiscv.＂I-214＂）基因组DNA为模板进行体外扩增,分离得到了MDCesAP、MXCesAP、JCesAP、YCesAP共4个片段。虽然4个片段序列之间及与美洲山杨中分离到的PtCesAP基因的同源性较低,但4个片断序列中的A/T含量都在60%-75%之间,且都有TATA box、poly（A）等启动子调控元件,因而有可能是新发现的特异启动子。

To obtain xylem specific promoters which can be used to control bole-boring pests, primers were designed according to PtCesA （ Populus tremuloid Michx） gene sequence. Four promoter-containing fragments, namely MDCesAP, MXCesAP, JCesAP and YCesAP, were amplified from genomic sequences of triploid Chinese white poplar-93 （Populus tomentosa Carr.-＂ 93＂）, Canada poplar-1 （Populus canadensis Moench. -＂1＂ ） and Italian poplar-214 （ Populus. canadensis cv. ＂1-214＂ ）, respectively. Sequence analysis revealed that their A ＋T content ranged from 60% to 75%, and they had TATA box, poly（A） signal and other promoter regulatory elements, suggesting that they are novel xylem-specific promoters. Moreover, the four fragments had low sequence identity with PtCesAP.