急性颅脑损伤患者血清中S100B蛋白的动态变化及临床意义

The dynamic change and clinical significance of serum of S100B protein in patients of acute craniocerebral injury

目的分析急性颅脑损伤患者血清中S100B蛋白的动态变化与疾病严重程度及预后的相关性。方法急性颅脑损伤患者60例按GCS评分分为轻型组10例,中型组35例,重型组15例。分别于发病后24 h内,第3、7、14天抽取空腹外周静脉血,使用双抗体夹心ELISA法测定S100B蛋白的血清浓度。结果3组S100B蛋白水平差异有统计学意义(P<0.01),即中型组S100B蛋白水平明显高于轻型组(P<0.01),重型组S100B蛋白水平明显高于轻、中型组(P<0.01)。结论S100B蛋白水平可以为急性颅脑损伤的早期诊断及预后评估提供有意义的信息。

Objective To analyze the relativity between dynamic change of S100B protein in serum and order of severity as well as the prognosis of patients with acute craniocerebral injury. Methods 60 patients with acute craniocerebral injury were enrolled as treatment groups, according to the GCS at admission, there were 10 mild cases ,35 moderate cases and 15 severe cases. 20 healthy subjects were enrolled as control group. The fasting peripheral blood was taken from vein,then serum concentrations of S100B protein were detected within 24 hours of onset, at 3 days, 7 days and 14 days after the attack. The serum levels of S100B protein were determined by Enzyme Linked Immuno Sorbent Assay （ELISA）. Results There was a significant differences between treatment groups and control group（ P 〈 0.01 ）. The levels of S100B protein in moderate cases were significantly higher than those of mild cases （ P 〈 0.01 ） , and the levels of S100B protein in severe cases were significantly higher than those of mild and moderate cases （ P 〈 0.01 ）. Conclusion Testing of the levels of S100B protein can play an important role in the early diagnosis and evaluation of prognosis for acute cranlocerebral injury.