原发性肝癌中肝细胞生长因子mRNA的定量表达及其临床意义

A clinical study on human HGF messenger RNA expression and its clinical implications in primary hepatocellular carcinoma

目的对原发性肝癌组织、癌旁组织以及正常肝脏组织肝细胞生长因子(HGF)mRNA进行定量测定,分析其表达与临床特征的关系,探讨其临床意义。方法采用实时荧光定量PCR检测59例原发性肝癌组织、相对应癌旁组织以及17例正常肝脏组织HGF mRNA的表达量,分析其表达量与性别、年龄、血清HBsAg、AFP、分化程度、肿瘤大小、临床分期、转移状态以及是否合并肝硬化等临床特征的关系。结果HGF mRNA在原发性肝癌组织、癌旁组织中的表达与正常肝脏组织差异无显著性(P>0.05)。HGF mRNA在肝癌合并肝硬化组中的表达明显高于未合并肝硬化组(P<0.05),但与其它临床特征无关。结论HGF mRNA在肝癌合并肝硬化组织中呈高表达,提示可能与肝癌发生发展有关。

Objective To investigate the expression of hepatocyte growth factor （HGF） mRNA and analyze whether HGF mRNA expression levels correlate with clinicopathologic variables in patients with primary hepatocellular carcinoma（HCC）. Methods Tissue samples, including 59 cancerous, adjacent non-cancerous tissues and 17 normal liver tissues were obtained from patients who underwent surgical resection. The expression of HGF mRNA was quantitatively analyzed by real - time PCR. Moreover, the relationship between HGF mRNA expression and clinicopathologic characters （such as sex, age, serum, HBsAg, AFP, differentiation degree, tumor size, clinical staging, metastasis, complicated with liver cirrhosis or not） were examined by statistical analysis. Results The expression of HGF mRNA in HCC patients complicated with liver cirrhosis was significantly higher than those without liver cirrhosis （ P 〈 0.05）. However, no significant difference was observed among HCC tissues, adjacent noncancerous and normal liver tissues （ P 〉 0.05）. There were no significant correlations between HGF mRNA and other clinicopathologic characters as well. Conclusions The HGF mRNA expression increased significantly in HCC patients complicated with liver cirrhosis, suggesting that it may be correlated with the development of HCC.