摘要
以玉米秸秆为基质,对15株白腐真菌进行了初步筛选,从中获得一株选择性系数较高的菌株Y10,经ITS-5.8S rDNA序列分析,初步鉴定为Cerrena sp.,并研究了该菌在30d培养期内降解玉米秸秆中木质纤维素的情况.结果表明,菌株Y10对玉米秸秆中木质素和半纤维素的降解速率明显高于纤维素;在30d的培养过程中,该菌对玉米秸秆降解的选择性系数都大于1,d15选择性系数最高为3.88.紫外光谱和红外光谱分析结果表明,经菌株Y10降解后玉米秸秆的化学成分发生了变化,且对木质素的降解程度要大于对纤维素的降解程度.
Fifteen white-rot fungi were screened with corn stalk used as degradation substrate. One white-rot fungus Y10 with high selectivity value was obtained, and it was identified as Cerrena sp. by ITS-5.8S rDNA sequences analysis. The degradation of corn stalk by Yl0 in 30 days was then studied. The results indicated that the speed of degrading lignin and hemicellulose by Y10 was faster than that of cellulose; the selectivity value was above 1 during the 30-day cultivation and the highest reached 3.88 after 15 days. The compositions of corn stalk before and after degradation were analyzed through ultraviolet spectroscopy and infrared spectroscopy, respectively. It was found that the degradation degree of lignin was greater than that of cellulose by Y10.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2009年第3期427-431,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家"863"计划项目(No.2007AA100702)
中国科学院项目(Nos.KSCX2-YW-G-008
K ZCX2-XB23-07-02)
国家科技支撑计划项目(Nos.2006BAD07A02
2006BAJ04B02)~~
关键词
白腐菌
玉米秸秆
选择性降解
木质素
纤维素
紫外光谱
红外光谱
white-rot fungus
corn stalk
selective degradation
lignin
cellulose
ultraviolet spectroscopy
infrared spectroscopy
